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首页> 外文期刊>Biotechnology Progress >Simultaneous biodegradation of benzene, toluene, and p-xylene in atwo-phase partitioning bioreactor: Concept demonstration and practicalapplication
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Simultaneous biodegradation of benzene, toluene, and p-xylene in atwo-phase partitioning bioreactor: Concept demonstration and practicalapplication

机译：两相分配生物反应器中苯，甲苯和对二甲苯的同时生物降解：概念验证与实际应用

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In this work, a mixture of benzene, toluene, and p-xylene was simultaneously biodegraded by Pseudomonas sp. ATCC 55595 in a two-phase partitioning bioreactor. This bioreactor consisted of a l-L cell-containing aqueous medium phase and a 500-mL immiscible organic phase. The organic solvent systematically selected for use in the bioreactor was Adol 85 NF, an industrial-grade, biocompatible solvent. In the first of three experiments, the organic phase was loaded with 2.0 g of benzene, 10.15 g of toluene, and 2.1 g of p-xylene, which partitioned into the aqueous phase at concentrations of 25, 50, and 8 mg/L, respectively. The system ultimately biodegraded all of the substrates within 144 h. During the rapid growth phase of this fermentation, the cells were oxygen-limited. This fermentation was therefore repeated using an enriched air supply to remove the oxygen limitation. The use of enriched air ultimately reduced the length of the fermentation to 108 h, thereby improving the overall volumetric consumption rates. Finally, 500 mt of Adol were used to recover 2.0 g of benzene, 10.15 g of toluene, and 2.1 g of p-xylene from silica sand that was contaminated as part of a simulated soil "spill". The solvent washing procedure was able to recover greater than 99% of each compound from the contaminated soil. The Adol was then transferred to the two-phase bioreactor to permit biological treatment of the BTX contaminants. This process was repeated when the initial BTX load had been consumed almost to exhaustion, and the solvent was able to recover the contaminants at greater than 99% efficiency once again. The system was ultimately able to degrade 4.0 g of benzene, 20.2 g of toluene, and 4.2 g of p-xylene within 144 h. These results represent an unprecedented level of BTX degradation and illustrate a potential practical application for this novel biotechnology.
机译：在这项工作中，假单胞菌（Pseudomonas sp。）同时降解了苯，甲苯和对二甲苯的混合物。两相分配生物反应器中的ATCC 55595。该生物反应器由含L-L细胞的水性介质相和500 mL不混溶的有机相组成。系统选择用于生物反应器的有机溶剂是工业级生物相容性溶剂Adol 85 NF。在三个实验的第一个实验中，有机相载有2.0 g苯，10.15 g甲苯和2.1 g对二甲苯，它们分别以25、50和8 mg / L的浓度分配到水相中，分别。该系统最终在144小时内对所有底物进行了生物降解。在该发酵的快速生长阶段，细胞受到氧气的限制。因此，使用富气供应重复该发酵以消除氧气限制。富空气的使用最终将发酵时间缩短至108小时，从而提高了总体积消耗率。最后，使用500公吨的Adol回收了作为模拟土壤“溢出物”的一部分而被污染的硅砂，回收了2.0 g苯，10.15 g甲苯和2.1 g对二甲苯。溶剂洗涤程序能够从受污染的土壤中回收大于99％的每种化合物。然后将Adol转移到两相生物反应器中，以对BTX污染物进行生物处理。当最初的BTX负载几乎耗尽时，重复此过程，并且溶剂能够再次以大于99％的效率回收污染物。该系统最终能够在144小时内降解4.0 g苯，20.2 g甲苯和4.2 g对二甲苯。这些结果代表了前所未有的BTX降解水平，并说明了这种新型生物技术的潜在实际应用。

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