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首页> 外文期刊>Biotechnology Progress >Production of acetone butanol ethanol (ABE) by a hyper-producing mutant strain of Clostridium beijerinckii BA101 and recovery by pervaporation
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Production of acetone butanol ethanol (ABE) by a hyper-producing mutant strain of Clostridium beijerinckii BA101 and recovery by pervaporation

机译：高产拜氏梭菌BA101的突变菌株生产丙酮丁醇乙醇（ABE），并通过全蒸发回收

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A silicone membrane was used to study butanol separation from model butanol solutions and fermentation broth. Depending upon the butanol feed concentration in the model solution and pervaporation conditions, butanol selectivities of 20.88-68.32 andflux values of 158.7-215.4 g m-2 h-1 were achieved. Higher flux values (400 g m-2 h-1) were obtained at higher butanol concentrations using air as sweep gas. In an integrated process of butanol fermentation-recovery, solvent productivities were improvedto 200% of the control batch fermentation productivities. In a batch reactor the hyper-butanol-producing mutant strain C. beijerinckii BA101 utilized 57.3 g/litre glucose and produced 24.2 g/litre total solvents, while in the integrated process it produced 51.5 g/litre (culture volume) total solvents. Concentrated glucose medium was also fermented. The C. beijerinckii BA101 mutant strain was not negatively affected by the pervaporative conditions. In the integrated experiment, acids were not produced. With the active fermentation broth, butanol selectivity was reduced by a factor of 2-3. However, the membrane flux was not affected by the active fermentation broth. The butanol permeate concentration ranged from 26.4 to 95.4 g/litre, depending upon butanol concentration in the fermentation broth. Since the permeate of most membranes contains acetone, butanol, and ethanol (and small concentrations of acids), it is suggested that distillation be used for further purification.
机译：有机硅膜用于研究从模型丁醇溶液和发酵液中分离丁醇。根据模型溶液中丁醇进料的浓度和全蒸发条件，丁醇的选择性为20.88-68.32，通量值为158.7-215.4 g m-2 h-1。使用空气作为吹扫气，在较高的丁醇浓度下可获得较高的通量值（400 g m-2 h-1）。在丁醇发酵-回收的集成过程中，溶剂生产率提高到对照分批发酵生产率的200％。在分批反应器中，产生高丁醇的突变菌株C.beijerinckii BA101使用57.3 g / L的葡萄糖，产生24.2 g / L的总溶剂，而在集成过程中，它产生51.5 g / L的总溶剂。浓缩的葡萄糖培养基也被发酵。拜氏梭菌BA101突变菌株不受渗透条件的负面影响。在综合实验中，未产生酸。使用活性发酵液，丁醇选择性降低了2-3倍。但是，膜通量不受活性发酵液的影响。取决于发酵液中丁醇的浓度，丁醇渗透物的浓度范围为26.4至95.4 g / L。由于大多数膜的渗透物包含丙酮，丁醇和乙醇（以及少量酸），因此建议使用蒸馏法进行进一步纯化。

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