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首页> 外文期刊>Biotechnology Progress >Penicillin G acylase-fatty lipid biocomposite films show excellent catalytic activity and long term stability/reusability
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Penicillin G acylase-fatty lipid biocomposite films show excellent catalytic activity and long term stability/reusability

机译：青霉素G酰基转移酶-脂肪生物复合膜具有出色的催化活性和长期稳定性/可重复使用性

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摘要

The formation of biocomposite films of the pharmaceutically important enzyme penicillin G acylase (PGA) and fatty lipids under enzyme-friendly conditions is described.The approach involves a simple beaker-based diffusion protocol wherein the enzyme diffuses into the lipid flilm during immersion in the enzyme solution,there by leading to the formation of a biocomposite film.The incorporation of the enzyme in both cationic as well as anionic lipids suggests the important role of secondary interactions such as hydrophobic and hydrogen bonding in the enzyme immobilization process.The kietics of formation of the enzyme-lipid biocomposites has been studied by quartz crystal microgravimentry (QCM) measurements.The stability of the enzyme in the lipid matrix was confirmed by Fourier transform infrared spectroscopy (FTIR)and biocatalytic activity measurements.Whereas the biological activity of the lipid-immobilized enzyme was marginally higher than that of the free enzyme,the biocomposiite film exhibited increased thermal/temporal stability.Particularly exciting was the observation that the biocomposite films could be reused in biocatalysis reactions with out significant loss inactivity,which indicates potentially exciting biomedical/industrial application of these films.
机译：描述了在酶友好条件下药物重要酶青霉素G酰基转移酶（PGA）和脂肪脂质的生物复合膜的形成方法，该方法涉及一种基于烧杯的简单扩散方案，其中酶在浸入酶过程中扩散到脂质膜中在阳离子和阴离子脂质中都掺入了酶，这表明在酶固定过程中次级相互作用（例如疏水和氢键）的重要作用。酶-脂质生物复合材料已通过石英晶体微重力（QCM）测量进行了研究。酶在脂质基质中的稳定性通过傅里叶变换红外光谱（FTIR）和生物催化活性测定得到了证实，而固定化脂质的生物活性酶比游离酶生物复合膜稍高表现出增加的热/时间稳定性。特别令人兴奋的是，观察到生物复合膜可以在生物催化反应中重复使用，而没有明显的失活，这表明这些膜在生物医学/工业上的潜在应用前景。

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