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首页> 外文期刊>AIDS Research and Human Retroviruses >Lack of high-level resistance mutations in HIV type 1 BF recombinant strains circulating in northeast Brazil.
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Lack of high-level resistance mutations in HIV type 1 BF recombinant strains circulating in northeast Brazil.

机译：在巴西东北部传播的HIV 1型BF重组菌株缺乏高水平的抗药性突变。

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Abstract The genetic variability and the prevalence of drug resistance-associated mutations (DRAM) of HIV-1 isolates from 50 women and 8 children from Feira de Santana, Bahia, Brazil were investigated. DNA samples were obtained and pol sequences were generated by PCR and direct sequencing. Phylogenetic analysis showed that 39 (67.2%) samples were subtype B, four (6.9%) F, one (1.7%) C, and 14 (24.1%) BF recombinants. Four different BF recombination patterns were detected. Twelve (20.7%) samples shared the same breakpoint within the reverse transcriptase (RT) sequence. Fifty-five (94.8%) isolates showed several resistance-associated mutations in the RT and the protease (PR) genes. Ten (17.2%) isolates presented mutations associated with a high level of resistance: nine (15.5%) to nucleoside RT inhibitors (NRTI), four (6.9%) to nonnucleoside RT inhibitors (NNRTI), and three (5.2%) to PR inhibitors (PIs). Subtype B-infected patients had, on average, 0.5 high-level DRAM per sequence while no mutations were observed in BF recombinants, although the two groups were under ARV for a similar period of time. Our data indicate the predominance of the subtype B, followed by BF recombinants in this population, and the dissemination of a recombinant strain in Bahia, which could be related to adaptive advantages of these variants over the predominant subtype B.
机译：摘要研究了来自巴西巴伊亚州费拉德桑塔纳的50名妇女和8名儿童的HIV-1分离株的遗传变异和耐药性突变（DRAM）的患病率。获得DNA样品，并通过PCR和直接测序产生pol序列。系统发育分析表明，有39个（67.2％）样品为B型，四个（6.9％）F，一个（1.7％）C和14个（24.1％）BF重组体。检测到四种不同的BF重组模式。十二（20.7％）个样本在逆转录酶（RT）序列内共享相同的断点。五十五（94.8％）分离株在RT和蛋白酶（PR）基因中显示了几个抗药性突变。十个（17.2％）分离株表现出与高水平抗药性相关的突变：九个（15.5％）对核苷RT抑制剂（NRTI），四个（6.9％）对非核苷RT抑制剂（NNRTI）和三个（5.2％）对PR抑制剂（PI）。尽管两个组处于ARV相似的时间段，但感染B型的患者平均每个序列具有0.5个高水平DRAM，而在BF重组体中未观察到突变。我们的数据表明，在该种群中B亚型占主导地位，其次是BF重组体，以及重组菌株在巴伊亚州的传播，这可能与这些变异体相对于B亚型的优势有关。

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