• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>AIDS Research and Human Retroviruses >Human T cell lymphotropic virus type 1 viral load variability and long-term trends in asymptomatic carriers and in patients with human T cell lymphotropic virus type 1-related diseases
【24h】

Human T cell lymphotropic virus type 1 viral load variability and long-term trends in asymptomatic carriers and in patients with human T cell lymphotropic virus type 1-related diseases

机译：人类T细胞淋巴病毒1型病毒载量变异性和无症状携带者以及人类T细胞淋巴病毒1型相关疾病患者的长期趋势

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Human T cell lymphotropic virus type 1 (HTLV-1) proviral load (PVL) in peripheral blood mononuclear cells (PBMCs) is high in patients with adult T cell leukemia/lymphoma or HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and in some asymptomatic carriers, but fluctuates. Our objectives were to document ranges of HTLV PVL across a broader spectrum of diseases and tissues, to quantify the normal range of intrapatient PVL variability, and to identify which PVL values and changes deserve further investigation. PVL was measured in 191 patients with HTLV-1-associated diseases and in 211 asymptomatic carriers, using real-time quantitative PCR. The intraassay variability increases as viral load decreases: 8% at high load, 17% at medium load, and 33% at low load. The interassay variability is not different from the intraassay. Mean intrapatient CV is 65% (SD 21) in asymptomatic carriers and 59% (SD 22) in HAM/TSP. PVL values varied widely between individuals, but were relatively constant within individuals. High PVL in cerebrospinal fluid (CSF) and lymph nodes (LN) was associated with disease but 57% of asymptomatic carriers had a PVL greater than 1% in PBMCs. Our results suggest that (1) PVL changes falling outside a coefficient of variation of 100% require more detailed assessment, (2) asymptomatic carriers with PVL higher than 10% should undergo more frequent clinical and laboratory monitoring, and (3) HTLV-1 PVL in blood and tissue is helpful in the diagnosis and monitoring of HTLV-1 infection.
机译：成人T细胞白血病/淋巴瘤或HTLV-1相关性脊髓病/热带痉挛性轻瘫（HAM /）的患者外周血单核细胞（PBMC）中的人类T细胞淋巴病毒1型（HTLV-1）前病毒载量（PVL）高TSP）和某些无症状的携带者，但会有所波动。我们的目标是记录更广泛的疾病和组织中的HTLV PVL范围，量化患者体内PVL变异性的正常范围，并确定哪些PVL值和变化值得进一步研究。使用实时定量PCR在191例HTLV-1相关疾病患者和211例无症状携带者中测量了PVL。随着病毒载量的减少，测定内变异性增加：高载量时为8％，中载量时为17％，低载量时为33％。批间差异与批内无差异。在无症状携带者中，平均住院患者CV为65％（SD 21），在HAM / TSP中为59％（SD 22）。 PVL值在个体之间差异很大，但在个体内部相对恒定。脑脊液（CSF）和淋巴结（LN）中的高PVL与疾病相关，但无症状携带者中有57％的PBMC中PVL大于1％。我们的结果表明（1）PVL的变化超出100％的变异系数需要更详细的评估;（2）PVL高于10％的无症状携带者应接受更频繁的临床和实验室监测;以及（3）HTLV-1血液和组织中的PVL有助于HTLV-1感染的诊断和监测。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号