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首页> 外文期刊>Biotechnology Progress >Stability of recombinant protein production in the GS-NSO expression system is unaffected by cryopreservation
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Stability of recombinant protein production in the GS-NSO expression system is unaffected by cryopreservation

机译：GS-NSO表达系统中重组蛋白生产的稳定性不受冷冻保存的影响

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摘要

Mammalian cells form a very important part of the repertoire of production systems available to scientists involved in the production of recombinant proteins. During the production of therapeutic proteins it is vital for regulatory approval of products that no phenotypic or genetic changes are observed in the cell line or product. As part of the generation and development of therapeutic protein production, cell lines have to be frozen at various stages to create cell banks. If cryopreservation and revival of frozen stocks were to give rise to any phenotypic changes in the cells, this would again be detrimental to the further development of that particular cell line. This study uses one of the most industrially important expression systems, the GS-NSO expression system, to examine the effect of cryopreservation on the growth and productivity profile of cell lines that exhibit differential degrees of stability during prolonged (production) culture periods. Results show that cryopreservation and revival procedures do not alter the stability characteristics of cell lines. This type of information is of great value in definition of protocols for cell line development.
机译：哺乳动物细胞构成了生产系统中非常重要的一部分，可供参与重组蛋白生产的科学家使用。在生产治疗性蛋白质的过程中，对于产品的监管批准至关重要的是，在细胞系或产品中未观察到表型或遗传变化。作为治疗性蛋白质生产的产生和发展的一部分，必须在各个阶段将细胞系冷冻以建立细胞库。如果冷冻保存和冷冻原种的复兴引起细胞的任何表型变化，这将再次不利于该特定细胞系的进一步发展。这项研究使用了最重要的工业表达系统之一，即GS-NSO表达系统，来研究冷冻保存对在延长的（生产）培养期间表现出不同程度的稳定性的细胞系生长和生产力状况的影响。结果表明，冷冻保存和恢复程序不会改变细胞系的稳定性。这种类型的信息对于细胞系开发协议的定义非常重要。

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