• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Porcine Pancreatic alpha-Amylase Hydrolysis of Native Starch Granules as a Function of Granule Surface Area
【24h】

Porcine Pancreatic alpha-Amylase Hydrolysis of Native Starch Granules as a Function of Granule Surface Area

机译：天然淀粉颗粒的猪胰α-淀粉酶水解与颗粒表面积的关系

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Porcine pancreatic alpha-amylase activity on native starch granules is more accurately described as a function of surface area of the granules rather than of substrate concentration. The apparent K_m of alpha-amylolysis of native starch from potato, maize, and rice expressed as a function of substrate concentration was largest for potato with a single value of V_max. However, the ratio of the slope of a Lineweaver-Burk plot to that of rice for enzymatic hydrolysis of native potato and maize starch were 7.78 and 2,58, respectively, which were very close to the ratio of surface area per mass of the two starch granules to that of rice. Therefore, the reciprocal of initial velocity was a linear function o fthe reciprocal of surface area for each starch granule. Surface area was calculated assuming the starch granules were spherical. The values obtained by this calculation were in good agreement with the value obtained by the photomicro-graphic method. By comparing enzymatic digestion of native maize granules to that of rice granules, it was concluded that the presence of pores in maize granules appeared to significantly affect overall rate of digetion after sufficient reaction time, but not at the very initial stage of hydrolysis.
机译：天然淀粉颗粒上的猪胰α-淀粉酶活性被更准确地描述为颗粒表面积的函数，而不是底物浓度的函数。马铃薯，玉米和大米中天然淀粉的α-淀粉分解的表观K_m（表示为底物浓度的函数）对于马铃薯最大，其单个值为V_max。但是，Lineweaver-Burk图的斜率与水稻的斜率之比分别为7.78和2,58，这两种酶用于天然马铃薯和玉米淀粉的酶水解，非常接近二者的表面积/质量比。淀粉粒比米粒。因此，初始速度的倒数是每个淀粉颗粒表面积的倒数的线性函数。假定淀粉颗粒为球形，计算表面积。通过该计算获得的值与通过显微照相法获得的值非常一致。通过比较天然玉米颗粒和大米颗粒的酶消化，可以得出结论，在足够的反应时间后，玉米颗粒中孔的出现似乎显着影响总的消化速率，但在水解的最初阶段却没有。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号