• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Comparctive Genomic Analysis fo dha Regulon and Related Genes for Anaerobic Glycerol Metabolism in Bacteria
【24h】

Comparctive Genomic Analysis fo dha Regulon and Related Genes for Anaerobic Glycerol Metabolism in Bacteria

机译：细菌厌氧甘油代谢的调节子和相关基因的比较基因组分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The dihydroxyacetone(dha) regulon of bacteria encodes genes for the anaerobic metabolism of glycerol.In this work,genomic data are used to analyze and compare the dha regulon and related genes in different organisms in silico with respect to gene organiation sequence similarity,and possible functions.Database searches showed that among the organisms,the genomes of thich have been sequenced so for,only two,i.e.,Kelbsiella pneumoniae MGH 78578 and Clostridium perfringens cfontain a complete dha regulon bearing all known enymes.The components adn their orpanization in the dha regulon of these two organisma siffer considerably from each other and also from the previously partially sequenzed dha regulons in Citrobacter freundii,CDlostridium pasterianum,and Clostridium hutyricum,Unlike all of the other organisms genes for the oxidative and reductive pathways of anaerobic glycerol metabolism in X.perfringesns are located in two separate organization units on the chromosome.CFomparisons of deduced protein sequences of genes with similar functions showed that the dha regulon components in K.penumoniae and C.Freundii have high similarities 980-95%) but lower similarities to those of the Clostridum species (30-80%) Interestingly,the protein sequence simialarities among the dha genes of the Clostriducm species are in many cased ever lower than those between the Clostridium species and D.pneumoniae or C freundii suggesting two different types of dha regulon in the Clostridium dpecies studied.The in silico reconstruction and comparison of dha regulons revealed several new genes in the microorganisms studied.In particular a novel dha kinase that is phosphoenolyruvate-dependent is identified and experi-mentally confirmed for K.pneumoniae in addition to the known ATP-dependent dha kinase This finding gives new insights into the regulation of glycerol metablolism in K.pneumoniae and explains some hitherto not well understood experimental observa-tions.
机译：细菌的二羟基丙酮（dha）调节子编码甘油的厌氧代谢基因。在这项工作中，利用基因组数据分析和比较了计算机中不同生物中的dha调节子和相关基因在基因组织序列相似性方面的可能性，并可能数据库搜索显示，在这些生物中，该菌的基因组已进行了测序，因此，只有两个，即肺炎克氏杆菌MGH 78578和产气荚膜梭状芽孢杆菌组成完整的dha调控区，带有所有已知的酶。这两个生物体的regulon彼此之间有很大的不同，也与先前在弗氏柠檬酸杆菌，巴斯德氏梭状芽胞杆菌和腐殖酸梭菌中部分分离的dha regulon有所不同，这与其他所有生物体中X厌氧甘油代谢的氧化和还原途径的基因不同。穿孔素位于染色体上两个独立的组织单元中。具有相似功能的基因的蛋白序列显示，K.penumoniae和C.Freundii中的dha regulon组分具有980-95％的高度相似性，但与梭菌属的相似性较低（30-80％）。有趣的是，该蛋白序列在许多情况下，梭菌属物种的dha基因之间的相似性一直低于梭菌属物种与肺炎克雷伯氏菌或C.freundii之间的相似性，这表明在梭状芽胞杆菌病中研究了两种不同类型的dha regulon。硅的计算机重建和dha regulons的比较揭示了研究的微生物中的几个新基因。特别是，除了已知的ATP依赖的dha激酶外，还鉴定并实验证实了肺炎克雷伯菌依赖的新型dha激酶，并通过实验证实了这一发现。肺炎克雷伯菌中的甘油代谢，并解释了一些迄今尚未完全了解的实验观察。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号