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首页> 外文期刊>Biotechnology Progress >Experimental and Theoretical Analysis of Tubular Membrane Aeration for Mammalian Cell Bioreactors
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Experimental and Theoretical Analysis of Tubular Membrane Aeration for Mammalian Cell Bioreactors

机译：哺乳动物细胞生物反应器管式膜通气的实验和理论分析

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A combination of experimental and theoretical approaches was used to characterize the dynamics of oxygen transfer in a membrane-aerated bioreactor. Pressure profiles along the length of the membrane at varying entrance and exit pressures were determined by actual experimental measurements, unlike most previous studies that have relied solely on theoretical descriptions of the pressure profile in the tubing. The mass transfer coefficient, k_La, was also determined under these conditions and was found to be essentially independent of tubing exit pressure. Measurement of the tubing pressure profile coupled with estimation of k_La allowed for computation of the oxygen transfre rate (OTR) along the length of the tubing. A mathematical model that incorporated friction pressure loss and losses due to tubing bending was developed to describe the pressure and hence OTR characteristics of membrane-aerated systems. The applicability of the model was verified by testing it on experimentally measured pressure data, and in all cases the model accurately described experimental data. When tubing properties are known, the mathematical model pressented in this study allows for a priori estimation of OTR profiles along the length of the tubing. This information is vital for optimal design and scale-up of membrane-aerated bioreactors for mammalian cell culture.
机译：实验方法和理论方法的组合被用来表征膜充气的生物反应器中氧气转移的动力学。沿膜的长度在变化的入口和出口压力下的压力分布是通过实际实验测量确定的，这与大多数以前的研究完全依赖于管中压力分布的理论描述不同。在这些条件下也确定了传质系数k_La，发现该系数基本上与管道出口压力无关。管道压力曲线的测量与k_La的估计相结合，可以计算沿管道长度的氧气透过率（OTR）。建立了包含摩擦压力损失和由于管弯曲而引起的损失的数学模型，以描述膜充气系统的压力及其OTR特性。通过在实验测量的压力数据上进行测试来验证模型的适用性，并且在所有情况下，模型均能准确描述实验数据。当已知油管特性时，本研究中采用的数学模型可以对油管长度上的OTR曲线进行先验估计。该信息对于优化设计和扩大用于哺乳动物细胞培养的膜曝气生物反应器至关重要。

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