Detection of low-frequency HIV type 1 reverse transcriptase drug resistance mutations by ultradeep sequencing in naive HIV type 1-infected individuals

BellecaveP.; Recordon-PinsonP.; PapuchonJ.; VandenhendeM.-A.; ReigadasS.; TauzinB.; FleuryH.

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Detection of low-frequency HIV type 1 reverse transcriptase drug resistance mutations by ultradeep sequencing in naive HIV type 1-infected individuals

机译：通过超深度测序检测初次感染HIV 1型个体的低频HIV 1型逆转录酶耐药性突变

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Genotypic resistance testing is recommended to evaluate the susceptibility of HIV to antiretroviral drugs. These tests are based on bulk population sequencing and thus consider only variants representing more than 20% of the viral population, whereas next generation sequencing methods allow detection below this threshold. We aimed to evaluate the potential use of ultradeep pyrosequencing (UDPS) for genotypic resistance testing in clinical routine at the University Hospital of Bordeaux, France. We performed UDPS on reverse transcriptase (RT) from 47 HIV-1 individuals, naive of antiretroviral treatment and for whom genotypic resistance testing was requested for clinical management in 2011-2012. In 8.5% of the patients, only low-frequency variants harboring RT drug resistance mutations were detected raising the question of their clinical significance. Rilpivirine-associated resistance mutations were detected in 19.1% of our population study. To conclude, UDPS could become a routine tool for the evaluation of HIV-infected patients in hospital laboratories.

机译：建议进行基因型耐药性测试，以评估HIV对抗逆转录病毒药物的敏感性。这些测试基于总体种群测序，因此仅考虑代表超过20％病毒种群的变异体，而下一代测序方法则允许在此阈值以下进行检测。我们旨在评估法国波尔多大学医院临床常规中超深焦磷酸测序（UDPS）在基因型耐药性检测中的潜在用途。我们对47位HIV-1个体的逆转录酶（RT）进行了UDPS，他们没有进行过抗逆转录病毒治疗，并且在2011-2012年要求对其进行基因型耐药性测试以进行临床管理。在8.5％的患者中，仅检测到具有RT耐药性突变的低频变异，这提出了其临床意义的问题。在我们的人口研究中，有19.1％的患者检出了与Rilpivirine相关的耐药性突变。总之，UDPS可以成为评估医院实验室中HIV感染患者的常规工具。

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《AIDS Research and Human Retroviruses》 |2014年第2期|共4页
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BellecaveP.; Recordon-PinsonP.; PapuchonJ.; VandenhendeM.-A.; ReigadasS.; TauzinB.; FleuryH.;
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1. Detection of low-frequency HIV type 1 reverse transcriptase drug resistance mutations by ultradeep sequencing in naive HIV type 1-infected individuals [J] . BellecaveP., Recordon-PinsonP., PapuchonJ., AIDS Research and Human Retroviruses . 2014,第2期

机译：通过超深度测序检测初次感染HIV 1型个体的低频HIV 1型逆转录酶耐药性突变
2. Profile of HIV Type 1 Infection and Genotypic Resistance Mutations to Antiretroviral Drugs in Treatment-Naive HIV Type 1-Infected Individuals in Hai Phong, Viet Nam [J] . Azumi Ishizaki, Nguyen Hung Cuong, Pham Van Thuc, AIDS Research and Human Retroviruses . 2009,第2期

机译：在越南海防市接受过治疗的未感染过HIV 1型感染的个体中的HIV 1型感染和抗逆转录病毒药物基因型耐药突变的概况
3. Characterization of HIV type 1 genotypes and drug resistance mutations among drug-naive HIV type 1-infected patients in Northern Vietnam. [J] . Phan TT, Ishizaki A, Phung D AIDS Research and Human Retroviruses . 2010,第2期

机译：在越南北部，未接受过HIV感染的1型艾滋病毒感染者中的HIV 1型基因型和耐药性突变的特征。
4. Predicting HIV-1 Protease and Reverse Transcriptase Drug Resistance Using Fuzzy Cognitive Maps [C] . Isel Grau, Gonzalo Napoles, Maria M. Garcia Iberoamerican congress on pattern recognition . 2013

机译：使用模糊认知图预测HIV-1蛋白酶和逆转录酶的耐药性
5. Do nucleoside analog-resistance mutations increase the fidelity of human immunodeficiency virus type-1 (HIV-1) reverse transcriptase? [D] . Curr, Kenneth Andrew. 2003

机译：核苷类似物抗性突变是否增加了人类1型免疫缺陷病毒（HIV-1）逆转录酶的保真度？
6. Positive Selection Detection in 40000 Human Immunodeficiency Virus (HIV) Type 1 Sequences Automatically Identifies Drug Resistance and Positive Fitness Mutations in HIV Protease and Reverse Transcriptase [O] . Lamei Chen, Alla Perlina, Christopher J. Lee 2004

机译：在40000种人类免疫缺陷病毒（HIV）1型序列中进行阳性选择检测可自动识别HIV蛋白酶和逆转录酶中的耐药性和正适应性突变
7. Positive Selection Detection in 40,000 Human Immunodeficiency Virus (HIV) Type 1 Sequences Automatically Identifies Drug Resistance and Positive Fitness Mutations in HIV Protease and Reverse Transcriptase [O] . Chen, Lamei, Perlina, Alla, Lee, Christopher J. 2004

机译：在40,000种人类免疫缺陷病毒（HIV）1型序列中进行阳性选择检测可自动识别HIV蛋白酶和逆转录酶中的耐药性和正适应性突变

Detection of low-frequency HIV type 1 reverse transcriptase drug resistance mutations by ultradeep sequencing in naive HIV type 1-infected individuals

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