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首页> 外文期刊>AIDS Research and Human Retroviruses >Profile of HIV Type 1 Infection and Genotypic Resistance Mutations to Antiretroviral Drugs in Treatment-Naive HIV Type 1-Infected Individuals in Hai Phong, Viet Nam
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Profile of HIV Type 1 Infection and Genotypic Resistance Mutations to Antiretroviral Drugs in Treatment-Naive HIV Type 1-Infected Individuals in Hai Phong, Viet Nam

机译：在越南海防市接受过治疗的未感染过HIV 1型感染的个体中的HIV 1型感染和抗逆转录病毒药物基因型耐药突变的概况

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摘要

We evaluated the prevalence and profile of antiretroviral treatment (ART)-associated resistance mutations among HIV-1 strains in northern Vietnam by genotypically analyzing strains isolated from ART-naive individuals in Hai Phong, a city in which HIV-1 is highly prevalent. Plasma samples were collected from injecting drug users (IDU, n=760), female sex workers (FSW, n=91), seafarers (n=94), pregnant women (n=200), and blood donors (n=210), and screened for HIV-1 antibodies. Plasma viral RNA was extracted from HIV-1-positive samples, amplified by reverse transcriptase (RT)-PCR of protease and RT genes, and analyzed for genotypes and ART-associated resistance mutations. HIV-1 prevalence among IDU, FSW, seafarers, pregnant women, and blood donors was 35.9%, 23.1%, 0%, 0.5%, and 2.9%, respectively. Phylogenetic analyses revealed that the most prevalent HIV-1 subtype was CRF01_AE (98.3%), similar to strains prevalent in southern China. Four (1.4%) subtype B strains and one (0.3%) unique recombinant between subtypes B and C were also identified. We found protease inhibitor-associated major resistance mutations in one of the 294 cases analyzed (0.3%; mutation M46I). We found RT inhibitor-associated major resistance mutations in 7/273 cases (2.6%; one occurrence each of L74I, M184I, and K219E; three cases of K103N; and two cases of G190E). One CRF01_AE strain harboring a protease codon 35 insertion was first identified in Vietnam. Thus, monitoring of drug-resistant HIV-1 and establishment of a database are required for the proper selection of ART in Vietnam.
机译：我们通过基因型分析从HIV-1高度流行的城市海防市从首次接受抗病毒治疗的个体中分离出的菌株，通过基因型分析，评估了越南北部HIV-1菌株中与抗逆转录病毒治疗（ART）相关的耐药突变的发生率和概况。从注射吸​​毒者（IDU，n = 760），女性性工作者（FSW，n = 91），海员（n = 94），孕妇（n = 200）和献血者（n = 210）中收集血浆样品，并筛选HIV-1抗体。从HIV-1阳性样本中提取血浆病毒RNA，通过蛋白酶和RT基因的逆转录酶（RT）-PCR进行扩增，并分析其基因型和与ART相关的耐药性突变。 IDU，FSW，海员，孕妇和献血者中的HIV-1流行率分别为35.9％，23.1％，0％，0.5％和2.9％。系统发育分析表明，最常见的HIV-1亚型为CRF01_AE（98.3％），与中国南部流行的菌株相似。还确定了四种（1.4％）B型亚型菌株和一种（0.3％）B型与C型之间的独特重组体。我们在分析的294例病例中发现了蛋白酶抑制剂相关的主要耐药突变（0.3％；突变M46I）。我们在7/273例中发现了与RT抑制剂相关的主要耐药突变（2.6％； L74I，M184I和K219E各自发生一次；三例K103N；以及两例G190E）。首先在越南鉴定出一种带有蛋白酶密码子35插入的CRF01_AE菌株。因此，在越南正确选择抗逆转录病毒治疗需要监测抗药性HIV-1和建立数据库。

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  • 来源
    《AIDS Research and Human Retroviruses》 |2009年第2期|175-182|共8页
  • 作者

    Azumi Ishizaki; Nguyen Hung Cuong; Pham Van Thuc; Nguyen Vu Trung; Kiyofumi Saijoh; Seiji Kageyama; Kyoko Ishigaki; Junko Tanuma; Shinichi Oka; Hiroshi Ichimura;

  • 作者单位

    Department of Viral Infection and International Health, Kanazawa University, Graduate School of Medical Science, Kanazawa, Japan.;

    Hai Phong Medical University, Hai Phong, Viet Nam.;

    Hai Phong Medical University, Hai Phong, Viet Nam.;

    Department of Medical microbiology, Hanoi Medical University, Hanoi, Viet Nam.;

    Department of Hygiene, Kanazawa University, Graduate School of Medical Science, Kanazawa, Japan.;

    Department of Viral Infection and International Health, Kanazawa University, Graduate School of Medical Science, Kanazawa, Japan.;

    AIDS Research and Clinical Center, International Medical Center of Japan, Tokyo, Japan.;

    AIDS Research and Clinical Center, International Medical Center of Japan, Tokyo, Japan.;

    AIDS Research and Clinical Center, International Medical Center of Japan, Tokyo, Japan.;

    Department of Viral Infection and International Health, Kanazawa University, Graduate School of Medical Science, Kanazawa, Japan.;

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