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首页> 外文期刊>AIDS Research and Human Retroviruses >Profile of HIV type 1 infection and genotypic resistance mutations to antiretroviral drugs in treatment-naive HIV type 1-infected individuals in Hai Phong, Viet Nam.
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Profile of HIV type 1 infection and genotypic resistance mutations to antiretroviral drugs in treatment-naive HIV type 1-infected individuals in Hai Phong, Viet Nam.

机译：在越南海防市，未接受过治疗的HIV 1型感染者中的HIV 1型感染和对抗逆转录病毒药物的基因型耐药突变的概况。

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摘要

We evaluated the prevalence and profile of antiretroviral treatment (ART)-associated resistance mutations among HIV-1 strains in northern Vietnam by genotypically analyzing strains isolated from ART-naive individuals in Hai Phong, a city in which HIV-1 is highly prevalent. Plasma samples were collected from injecting drug users (IDU, n = 760), female sex workers (FSW, n = 91), seafarers (n = 94), pregnant women (n = 200), and blood donors (n = 210), and screened for HIV-1 antibodies. Plasma viral RNA was extracted from HIV-1-positive samples, amplified by reverse transcriptase (RT)-PCR of protease and RT genes, and analyzed for genotypes and ART-associated resistance mutations. HIV-1 prevalence among IDU, FSW, seafarers, pregnant women, and blood donors was 35.9%, 23.1%, 0%, 0.5%, and 2.9%, respectively. Phylogenetic analyses revealed that the most prevalent HIV-1 subtype was CRF01_AE (98.3%), similar to strains prevalent in southern China. Four (1.4%) subtype B strains and one (0.3%) unique recombinant between subtypes B and C were also identified. We found protease inhibitor-associated major resistance mutations in one of the 294 cases analyzed (0.3%; mutation M46I). We found RT inhibitor-associated major resistance mutations in 7/273 cases (2.6%; one occurrence each of L74I, M184I, and K219E; three cases of K103N; and two cases of G190E). One CRF01_AE strain harboring a protease codon 35 insertion was first identified in Vietnam. Thus, monitoring of drug-resistant HIV-1 and establishment of a database are required for the proper selection of ART in Vietnam.
机译：我们通过基因型分析从海防（一个HIV-1高度流行的城市）的从未接受过ART治疗的个体中分离出的菌株，对越南北部HIV-1菌株中抗逆转录病毒治疗（ART）相关的耐药突变的发生率和概况进行了评估。从注射吸​​毒者（IDU，n = 760），女性性工作者（FSW，n = 91），海员（n = 94），孕妇（n = 200）和献血者（n = 210）中收集血浆样品，并筛选HIV-1抗体。从HIV-1阳性样本中提取血浆病毒RNA，通过蛋白酶和RT基因的逆转录酶（RT）-PCR进行扩增，并分析其基因型和与ART相关的耐药性突变。 IDU，FSW，海员，孕妇和献血者中的HIV-1流行率分别为35.9％，23.1％，0％，0.5％和2.9％。系统发育分析表明，最常见的HIV-1亚型为CRF01_AE（98.3％），与中国南部流行的菌株相似。还鉴定了四种（1.4％）B型亚型菌株和一种（0.3％）B型与C型之间的独特重组体。我们在分析的294例病例中发现了蛋白酶抑制剂相关的主要耐药突变（0.3％;突变M46I）。我们在7/273例中发现了与RT抑制剂相关的主要耐药突变（2.6％; L74I，M184I和K219E各自发生一次; K103N发生三例; G190E发生了两例）。首先在越南鉴定出一种带有蛋白酶35密码子插入的CRF01_AE菌株。因此，在越南正确选择抗逆转录病毒治疗需要监测抗药性HIV-1和建立数据库。

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