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首页> 外文期刊>Toxicology Letters: An International Journal Providing a Forum for Original and Pertinent Contributions in Toxicology Research >Constitutive expression of cytochrome P450 in foetal and adult porcine livers-Effects of body weight
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Constitutive expression of cytochrome P450 in foetal and adult porcine livers-Effects of body weight

机译：细胞色素P450在胎儿和成人猪肝肝的组成型表达 - 体重的影响

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摘要

The liver hosts a great number of enzymatically driven processes, including detoxification. The super-family of enzymes named cytochrome P450 (CYP) is the major participant in that process. The expression of CYPs is affected by several factors including life-stage (foetal vs. adult). In the present study we investigated the impact of birth-weight (high or low birth weight) and life-stage on constitutive expression of porcine hepatic CYP1A1, CYP1A2, CYP2A19, CYP2B22, CYP2C33, CYP2D25, CYP2E1 and CYP3A29, as well as the transcription factors controlling their expression; aryl hydrocarbon receptor, constitutive androstane receptor, pregnane X receptor, C/EBP and hepatocyte nuclear factors 1 and 4. Both RT-PCR and western blotting showed a marked increase in the expression of the adult pigs compared with prenatal pigs. Moreover, CYP2E1 mRNA expression was 7.5 fold higher in foetuses with low birth weight compared with foetuses with high birth weight. Gender did not affect the mRNA expression within the different life-stages. These results indicate a similarity to what is observed in humans and porcine foetuses may therefore be a model for humans when studying expression of CYPs. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
机译：肝脏占多数酶促驱动的过程，包括解毒。称为细胞色素P450（CYP）的酶的超级族是该过程的主要参与者。 Cyps的表达受若干因素，包括生命阶段（胎儿与成人）。在本研究中，我们研究了猪肝CYP1A1，CYP1A2，CYP2A19，CYP2B22，CYP2C33，CYP2D25，CYP2E1和CYP3A29的组成型表达对猪肝CYP1A1，CYP1A2，CYP2A19，CYP2B2229，CYP2E1和CYP3A29的影响，以及转录的影响控制他们表达的因素;芳基烃受体，组成型和过甲烷受体，妊娠X受体，C / EBP和肝细胞核因子1和4。RT-PCR和Western印迹显示出成年猪的表达显着增加，与产前猪相比。此外，与具有高出生体重高的胎儿相比，CYP2E1 mRNA表达具有低7.5倍。性别没有影响不同寿命内的mRNA表达。这些结果表明，当研究Cyps的表达时，对人类和猪胎儿中观察到的相似性可能是人类的模型。 （c）2016 Elsevier Ireland Ltd.保留所有权利。

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