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首页> 外文期刊>Toxicology Letters: An International Journal Providing a Forum for Original and Pertinent Contributions in Toxicology Research >Development of an in vitro method to estimate the sensitization induction level of contact allergens
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Development of an in vitro method to estimate the sensitization induction level of contact allergens

机译：估计接触过敏原的敏化诱导水平的体外方法的发展

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No standardized in vitro methods to assess potency of skin sensitizers are available. Recently, we standardized a procedure which combines the epidermal equivalent potency assay with assessment of IL18 to provide a single test for identification and classification of skin sensitizers. This current study aimed to extend tested chemicals, and to provide a simple in vitro method for estimation of the expected sensitization induction level interpolating in vitro EC50 and IL-18 SI2 values to predict LLNA EC3 and/or human NOEL from standards curves generated using reference contact allergens. Reconstituted human epidermis was challenged with 14 chemicals not previously tested benzoquinone, chlorpromazine, chloramine T, benzyl salicylate, diethyl maleate, dihydroeugenol, 2,4-dichloronitrobenzene, benzyl cinnamate, imidazolidinyl urea, and limonene as contact sensitizers while benzyl alcohol, isopropanol, dimethyl isophthalate and 4-aminobenzoic acid as non-sensitizers in the LLNA. Where for benzyl salicylate and benzyl cinnamate no sensitization was observed in human predictive studies, positive responses to benzyl alcohol and dimethyl isophthalate were reported. The proposed method correlates better with human data, correctly predicting substances incorrectly classified by LLNA. With the exception of benzoquinone (interference with both MTT and IL-18 ELISA), and chloramine T (underestimated in the interpolation), a good estimation of LLNA EC3 and in vivo available human NOEL values was obtained. (C) 2017 Elsevier B.V. All rights reserved.
机译：没有标准化的体外方法，以评估皮肤敏感剂的效力。最近，我们标准化了一种程序，该程序将表皮等同效力测定与IL18评估结合，以提供单一的鉴定和分类皮肤敏感剂的测试。本前研究旨在延伸测试化学品，并提供一种简单的体外方法，用于估计内插体外EC50和IL-18 Si2值的预期致敏感应水平，以预测使用参考产生的标准曲线的LLNA EC3和/或人Noel接触过敏原。重构的人表皮面临着14种化学物质以前未测试的苯醌，氯丙嗪，氯胺T，苄基，马来酸二甲基，二羟基苯酚，2,4-二氯苯苯甲苯，苄基，咪唑烷基脲和柠檬烯作为接触敏化剂，而苄醇，异丙醇，二甲基苯二酚邻苯二甲酸酯和4-氨基苯甲酸作为LLNA中的非敏化剂。在人类预测研究中观察到苄基水杨酸苄酯和苄基没有敏化，据报道对苄醇和苯甲酸二甲酯的正反应。所提出的方法对人类数据的相关性更好地相关，正确预测LLNA归类不正确的物质。除苯醌（干扰MTT和IL-18 ELISA）和氯胺T（在插值中低估）外，获得了LLNA EC3和体内可用人塔尔值的良好估计。 （c）2017 Elsevier B.v.保留所有权利。

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