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首页> 外文期刊>Transfusion medicine and hemotherapy: offizielles Organ der Deutschen Gesellschaft fur? Transfusionsmedizin und Immunham?atologie >Reactive Oxygen Species Generated by CD45-Cells Distinct from Leukocyte Population in Platelet Concentrates Is Correlated with the Expression and Release of Platelet Activation Markers during Storage
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Reactive Oxygen Species Generated by CD45-Cells Distinct from Leukocyte Population in Platelet Concentrates Is Correlated with the Expression and Release of Platelet Activation Markers during Storage

机译：在血小板浓缩物中不同于白细胞群不同的CD45细胞产生的反应性氧物种与储存期间血小板活化标志物的表达和释放相关

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Background: Platelet stimulation with agonists is accompanied by the generation of reactive oxygen species (ROS) which promotes further platelet activation and aggregation. Considering different cell populations in platelet concentrates (PCs), this study investigates the correlation of ROS generation with the expression and release of platelet activation markers during storage. Methods: Samples obtained from 6 PCs were subjected to flow cytometry and ELISA to evaluate the expression and shedding of platelet P-selectin or CD40L during storage. Intracellular ROS were detected in either CD45- or CD45+ population by flow cytometry using dihydrorhodamine 123, while ROS production was analyzed in both P-selectin+ or P-selectin-and CD40L+ or CD40L-populations. To further evaluate the correlation between ROS generation and release function, TRAP-stimulated platelets were also subjected to flow cytometry analysis. Results: ROS detected in the CD45-population (leukocyte-free platelets) was significantly increased by fMLP and PMA. P-selectin-or CD40L-platelet did not show significant amount of ROS. Total ROS generation was significantly increased during platelet storage (day 0 vs. day 5; p = 0.0002) while this increasing pattern was directly correlated with the expression of P-selectin (r = 0.72; p = 0.0001) and CD40L (r = 0.69; p = 0.0001). ROS generations were significantly correlated with ectodomain shedding of these pro-inflammatory molecules. Conclusion: Our data confirmed increasing levels of intracellular ROS generation in both platelets (CD45-) and platelet-leukocyte aggregates (CD45+) during PC storage. The amount of detected ROS is directly correlated with platelet activation and release in each population while platelet-leukocyte aggregates generate higher levels of ROS than single platelets. (c) 2018 S. Karger GmbH, Freiburg
机译：背景：用激动剂的血小板刺激伴随着产生的反应性氧物种（ROS），其促进进一步的血小板活化和聚集。考虑到血小板浓缩物（PCS）中的不同细胞群，本研究研究了ROS生成与储存期间血小板活化标志物的表达和释放的相关性。方法：对6次PC的样品进行流式细胞术和ELISA，以评估储存过程中血小板p-选择蛋白或CD40L的表达和脱落。通过使用二氢胺胺123，通过流式细胞术检测细胞内ROS，在CD45-或CD45 +群体中检测，而在P-SELETIN +或P-SELETIN-AND CD40L +或CD40L-群体中分析ROS产生。为了进一步评估ROS生成和释放功能之间的相关性，还对陷阱刺激的血小板进行流式细胞术分析。结果：FMMP和PMA中检测到CD45人口（无白细胞血小板）中检测到的ROS。 p-selectin-or cd40l-血小板没有显示大量的ROS。血小板储存过程中的总ROS生成显着增加（第5天，第5天; P = 0.0002），而这种增加的模式与p-选择素的表达直接相关（r = 0.72; p = 0.0001）和CD40L（r = 0.69 ; p = 0.0001）。 ROS世代与这些促炎分子的外胚层脱落显着相关。结论：我们的数据证实了在PC储存期间血小板（CD45-）和血小板 - 白细胞聚集体（CD45 +）中的细胞内ROS产生的增加。检测到的RO的量与每种群体中的血小板活化和释放直接相关，而血小板白细胞聚集体产生比单个血小板更高水平的RO。 （c）2018年S. Karger GmbH，Freiburg

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