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首页> 外文期刊>Alcoholism: Clinical and experimental research >Chronic ethanol feeding alters hepatocyte memory which is not altered by acute feeding.
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Chronic ethanol feeding alters hepatocyte memory which is not altered by acute feeding.

机译：慢性乙醇喂养会改变肝细胞记忆，急性喂养不会改变这种记忆。

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BACKGROUND: Gene expression changes in the liver after acute binge drinking may differ from the changes seen in chronic ethanol feeding in the rat. The changes in gene expression after chronic ethanol feeding may sensitize the liver to alcohol-induced liver damage, which is not seen after acute binge drinking. METHODS: To test this hypothesis, gene microarray analysis was performed on the livers of rats (n = 3) fed an acute binge dose of ethanol (6 g/kg body wt) and killed at 3 and 12 hours after ethanol by gavage. The gene microarrays were compared with those made on the liver of rats from a previous study, in which the rats were fed ethanol by intragastric tube for 1 month (36% of calories derived from ethanol). RESULTS: Microarray analysis data varied between the acute and chronic models in several important respects. Growth factors increased mainly in the chronic alcohol fed rat. Changes in enzymes involved in oxidative stress were noted only with chronic ethanol feeding. Gene expression of fat metabolism was increased only with chronic ethanol feeding. Most importantly, epigenetic related enzymes and acetylation and methylation of histones changed only after chronic ethanol feeding. CONCLUSIONS: The results support the concept that chronic ethanol ingestion induces altered gene expression as a result of changes in epigenetic mechanisms, where acetylation and methylation of histones were altered.
机译：背景：急性暴饮暴食后肝脏中的基因表达变化可能与大鼠长期饮酒中观察到的变化不同。长期喂食乙醇后基因表达的变化可能使肝脏对酒精引起的肝损伤敏感，急性暴饮后未见此现象。方法：为了验证该假设，对大鼠（n = 3）急性暴饮暴食剂量的乙醇（6 g / kg体重）的肝脏进行了基因微阵列分析，并在分别用乙醇强饲后3和12小时将其杀死。将该基因芯片与先前研究中在大鼠肝脏上制备的基因芯片进行了比较，在该研究中，大鼠通过胃内导管喂食了乙醇1个月（36％的热量来自乙醇）。结果：芯片模型分析数据在急性和慢性模型之间在几个重要方面有所不同。生长因子主要在长期饮酒的大鼠中增加。仅在长期喂食乙醇的情况下才注意到与氧化应激有关的酶的变化。脂肪代谢的基因表达仅通过长期乙醇喂养才能增加。最重要的是，表观遗传相关的酶以及组蛋白的乙酰化和甲基化只有在长期喂食乙醇后才发生变化。结论：结果支持这样的概念，即由于表观遗传机制的改变，慢性乙醇摄入会诱导基因表达的改变，其中组蛋白的乙酰化和甲基化被改变。

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