>As the prospect of human beings exposed to new nanomaterials increases, there are growing concerns about the foetal exposure and effects of such nanomate'/> Iron‐related toxicity effects of single‐walled carbon nanotubes in human placental cells ( <fc >BeWo</fc>BeWo ) investigated by X‐ray fluorescence microscopy
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首页> 外文期刊>X-Ray Spectrometry: An International Journal >Iron‐related toxicity effects of single‐walled carbon nanotubes in human placental cells ( BeWoBeWo ) investigated by X‐ray fluorescence microscopy
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Iron‐related toxicity effects of single‐walled carbon nanotubes in human placental cells ( BeWoBeWo ) investigated by X‐ray fluorescence microscopy

机译：用X射线荧光显微镜研究人胎盘细胞单壁碳纳米管（ Bewo Bewo）的铁相关毒性效应

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>As the prospect of human beings exposed to new nanomaterials increases, there are growing concerns about the foetal exposure and effects of such nanomaterials during pregnancy. Among others, due to their ample usage, it becomes urgent to address the vulnerability of the human placental barrier to carbon nanotubes (CNTs). Recently, by applying a combination of advanced synchrotron‐based X‐ray microscopy and X‐ray fluorescence (XRF) techniques, we demonstrated that raw single‐walled CNTs (SWCNTs) may cause an asbestos‐like iron‐related toxicity in mesothelial (MeT5A) cells. This work shows the results obtained with the same approach and experimental conditions in human choriocarcinoma (BeWo) cells as a placental model. XRF microscopy in mild and soft X‐ray regimes was used to map the distribution of P, S, Ca, and Fe in exposed and control cells, whereas absorption and phase‐contrast imaging (soft X‐ray microscopy) provided simultaneous morphological information. The findings show that exposure to SWCNTs affects cell viability and causes a small increase in intracellular iron content, further confirmed by the stimulation of the ferritin protein. It is interesting to note that the iron changes are negligible when highly purified SWCNTs are used. In all tested conditions, including exposure to iron sulfate, the iron accumulation or ferritin increase was much lower in BeWo cells than in MeT5A ones, suggesting a different iron metabolism and possibly a lower vulnerability of the placental barrier to iron‐rich nanomaterials.
机译：

作为暴露于新的纳米材料的人类的前景增加，对妊娠期间胎儿暴露和这种纳米材料的影响日益令人担忧。其中，由于它们的使用量充足，因此解决人体胎盘屏障对碳纳米管（CNT）的脆弱性迫切需要。最近，通过应用基于先进的同步氧化物的X射线显微镜和X射线荧光（XRF）技术的组合，我们证明了原始的单壁CNT（SWCNTS）可能导致间皮（Met5a）中的石棉相关的铁相关毒性。 ） 细胞。该作品表明，在人丛生癌（Bewo）细胞中具有与胎盘模型相同的方法和实验条件获得的结果。使用温和和软X射线制度中的XRF显微镜用于映射暴露和控制细胞中P，S，Ca和Fe的分布，而吸收和相位对比度成像（软X射线显微镜）提供了同时形态学信息。结果表明，暴露于SWCNTS影响细胞活力并导致细胞内铁含量的小增加，通过刺激铁蛋白蛋白进一步证实。有趣的是，当使用高度纯化的SWCNT时，铁变化可忽略不计。在所有测试条件下，包括暴露于硫酸铁的情况下，Bewo细胞中的铁累积或铁蛋白增加远低于met5a，暗示不同的铁代谢，并且可能是胎盘屏障对铁的纳米材料的更低脆弱性。

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