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首页> 外文期刊>Tropical Animal Health and Production >Detection ofBrucellaspp. in raw milk from various livestock species raised under pastoral production systems in Isiolo and Marsabit Counties, northern Kenya
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Detection ofBrucellaspp. in raw milk from various livestock species raised under pastoral production systems in Isiolo and Marsabit Counties, northern Kenya

机译：检测BrucentApp。 在来自斯蒂洛北部北肯尼亚的田园田间牧草生产系统下，在各种牲畜种类中提出的原料牛奶

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Introduction Brucellosis is an important zoonotic disease in Kenya, and identifying the bacteria in milk is important in assessing the risk of exposure in people. Methods A cross-sectional study that involved 175 households was implemented in the pastoral counties of Marsabit and Isiolo in Kenya. Pooled milk samples (n = 164) were collected at the household level, and another 372 were collected from domesticated lactating animals (312 goats, 7 sheep, 50 cattle and 3 camels). Real-time polymerase chain reaction (qPCR) testing of the milk samples was performed to identify Brucella species. Brucella anti-LPS IgG antibodies were also detected in bovine milk samples using an indirect enzyme-linked immunosorbent assay (ELISA). Results Based on the qPCR, the prevalence of the pathogen at the animal level (considering samples from individual animals) was 2.4% (95% confidence interval (CI) 1.1-4.5) and 3.0% (CI: 1.0-7.0) in pooled samples. All 14 samples found positive by qPCR were from goats, with 10 contaminated with B. abortus and 4 with B. melitensis. The Brucella spp. antibody prevalence in bovine milk using the milk ELISA was 26.0% (95% CI: 14.6-40.3) in individual animal samples and 46.3% (95% CI: 30.7-62.6) in pooled samples. Conclusion The study is the first in Kenya to test for Brucella spp. directly from milk using qPCR without culturing for the bacteria. It also detected B. abortus in goats, suggesting transmission of brucellosis between cattle and goats. The high prevalence of Brucella spp. is a significant public health risk, and there is a need for intervention strategies necessary in the study area.
机译：引言布鲁氏菌病是肯尼亚的重要动物疾病，并鉴定牛奶中的细菌对于评估人们暴露的风险很重要。方法涉及175户家庭的横断面研究在肯尼亚马萨比斯和Isiolo的田园县实施。在家庭水平收集汇集的牛奶样品（n = 164），并从驯化哺乳动物（312只山羊，7只羊，50牛和3只骆驼）中收集另外372个。进行实时聚合酶链反应（QPCR）乳汁样品的测试以鉴定布鲁氏菌。使用间接酶联免疫吸附测定（ELISA），还在牛奶样品中检测到Brucella抗LPS IgG抗体。基于QPCR的结果，动物水平的病原体的患病率（考虑各自动物的样品）为2.4％（95％置信区间（CI）1.1-4.5）和3.0％（CI：1.0-7.0）在合并的样品中。所有14个样品通过QPCR发现阳性来自山羊，10种污染的B. abortus和4种带B. melitensis。布鲁氏菌SPP。使用牛奶牛奶的抗体患病率在牛奶中的26.0％（95％CI：14.6-40.3），在合并样品中，46.3％（95％CI：30.7-62.6）。结论该研究是肯尼亚的第一个测试Brucella SPP。使用QPCR直接来自牛奶而不为细菌培养。它还检测到山羊的B. Abortus，建议在牛和山羊之间传播布鲁曲。 Brucella SPP的高患病率。是一个重要的公共卫生风险，需要在研究领域有必要的干预策略。

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