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Foot-and-mouth disease in Southern Ghana: occurrence and molecular characterization of circulating viruses

机译：加纳南部的脚口病：循环病毒的发生和分子表征

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Foot-and-mouth disease (FMD) is considered to be endemic in Ghana. However, our knowledge of the local epidemiology of the disease is restricted by a lack of serological information and data for characterized viruses causing field outbreaks. In order to improve our understanding of the prevailing situation, this study was initiated to establish the FMD viruses (FMDV) circulating in the country. During 2016, sera (n=93) and epithelia/oral swab (n=20) samples were collected from cattle from four districts in Southern Ghana that experienced FMD outbreaks. Sera were analyzed using the PrioCHECK (R) FMDV non-structural protein (NSP) ELISA whereas the epithelia/oral swab samples were examined by virus isolation, antigen ELISA, reverse transcription polymerase chain reaction (RT-PCR), and sequencing of VP1 followed by phylogenetic analysis. Assay for antibodies against FMDV NSPs provided evidence of exposure to FMDV in 88.2% (82/93) of the sera tested. Serotypes O and A viruses were detected from clinical samples by RT-PCR and sequencing of VP1. Phylogenetic analysis of VP1 coding sequences revealed that the serotype O viruses belonged to the West Africa (WA) topotype and were most closely related to viruses from Niger and Benin, while the serotype A viruses clustered within genotype IV (G-IV) of the Africa topotype and were most closely related to viruses from Nigeria. This study provides useful information on FMDV serotypes and viral lineages that circulate in Ghana and West Africa that may aid in the formulation of effective FMD control strategies.
机译：口蹄疫（FMD）被认为是加纳的地方。然而，我们对疾病的当地流行病学的了解受到缺乏血清学信息和具有引起现场爆发的病毒的数据的限制。为了提高我们对普遍情况的理解，启动了该研究以建立在该国流通的FMD病毒（FMDV）。在2016年期间，从南加纳的四个地区的牛收集血清（n = 93）和上皮/口服拭子（n = 20）样品，经历了FMD爆发。使用PrioCheck（R）FMDV非结构蛋白（NSP）ELISA分析血清，而上皮/口腔拭子样品被病毒分离检查，抗原ELISA，逆转录聚合酶链反应（RT-PCR）以及vp1的序列通过系统发育分析。针对FMDV NSP的抗体的测定提供了在测试的88.2％（82/93）中暴露于FMDV的证据。通过RT-PCR从临床样品中检测血清型o和病毒，并对VP1的测序进行测序。 VP1编码序列的系统发育分析显示，血清型O病毒属于西非（WA）拓扑型，与尼日尔和贝宁的病毒最密切相关，而血清型在非洲基因型IV（G-IV）中聚集在一起拓扑型并与尼日利亚病毒最密切相关。本研究提供有关在加纳和西非流通的FMDV血清型和病毒谱系的有用信息，可有助于制定有效的FMD控制策略。

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