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首页> 外文期刊>Tropical Animal Health and Production >Genetic polymorphisms in part of intron 7 and exon 8 of HSP90AA1 gene and its association with heat tolerance traits in two exotic layer chicken strains
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Genetic polymorphisms in part of intron 7 and exon 8 of HSP90AA1 gene and its association with heat tolerance traits in two exotic layer chicken strains

机译：HSP90AA1基因内含子7和外显子8的遗传多态性及其与两种异乎寻常的鸡菌株的耐热性关系

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摘要

Chickens, like other vertebrates, react to stress conditions through their cultured cells by expressing heat shock proteins (HSPs). Genetic association of single-nucleotide polymorphisms (SNPs) in HSPs with desirable traits will reveal their importance as potential genetic markers. Blood samples were collected from 50 birds per strain for DNA extraction, polymerase chain reaction amplification and sequencing of the HSP90AA1 gene. SNPs were detected using Codoncode Aligner. Association of each SNP with heat tolerance traits was analysed using generalized linear model procedure of SAS. A total of seven SNPs were detected, four SNPs; A7T, A160T, T223A and C134T were detected in part of intron 7 to exon 8 of HSP90AA1 gene of BRD while three A160T, T223A and C134T were detected in HYL. SNPC134T, a synonymous variant, was detected in exon 8. Only SNPA7T was in Hardy-Weinberg equilibrium (X-2 = 0.03) but had no association with the traits measured. Polymorphic information content calculated showed SNPA160T to be moderately polymorphic; other SNPs were lowly polymorphic. Heterozygosity for SNPs-A160T and T223A of BRD showed moderate genetic variation while the other SNPs and those in HYL recorded low genetic variation. The study concluded that the SNPs detected were majorly lowly polymorphic and also the SNP locus A7T in intron 7 of HSP90AA1 of BRD had no genetic association with heat tolerance traits.
机译：与其他脊椎动物一样，鸡通过表达热休克蛋白（HSP）通过培养的细胞反应应激状况。 HSP中具有所需性状的单核苷酸多态性（SNP）的遗传结合将揭示其视为潜在的遗传标志物的重要性。从每种菌株50只鸟收集血液样品，用于DNA提取，聚合酶链反应扩增和HSP90AA1基因的测序。使用Codoncode对准器检测SNP。使用SAS的广义线性模型程序分析每个SNP具有耐热性状的每个SNP。共检测到七个SNP，四个SNPS;在BRD的HSP90AA1基因的一部分内检测到A7T，A160T，T223A和C134T，在HSP90AA1基因的EXON 8中检测，而在Hyl中检测到三个A160T，T223A和C134T。 SNPC134T是一个同义变体，在外显子8中被检测到。只有SNPA7T在Hardy-Weinberg平衡（X-2 = 0.03）中，但没有与测量的特征相关联。计算的多态信息含量显示SNPA160T是适度的多态;其他SNP是低多态性的。 BRD的SNPS-A160T和T223A的杂合性显示出中等的遗传变异，而其他SNP和Hyl记录的低遗传变异。该研究得出结论，检测到的SNP是主要的低多态性，并且BRD的HSP90AA1的Intron 7中的SNP基因座A7T没有遗传关联与耐热性状。

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