• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Tropical Animal Health and Production >Report of outbreaks of classical scrapie in Dorper sheep and associated prion protein gene polymorphisms in affected flocks
【24h】

Report of outbreaks of classical scrapie in Dorper sheep and associated prion protein gene polymorphisms in affected flocks

机译：受影响的羊毛中的Dorper绵羊和相关朊病毒蛋白基因多态性爆发的报告

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Scrapie is an infectious neurodegenerative disease affecting sheep and goats, related with conformational alteration of an isoform of the prion protein that leads to deposition and aggregation in the host's central nervous system. Occurrence of the natural disease can be influenced by host genetic factors, such as a single nucleotide polymorphism of the prion protein gene. This study reports three scrapie-affected Dorper flocks located on three different farms in Brazil. The objective of this study was to analyze these three flocks using scrapie diagnostics, combining histology, immunohistochemistry, genotyping, and western blot assays. For immunohistochemistry, 192 sheep were selected and 308 sheep blood samples were taken for genotyping. A total of 22 sheep were scrapie positive by immunohistochemistry. Of these, four presented clinical signs and had scrapie immunoreactivity at the obex in western blot assays. The sheep without clinical signs were positive in lymphoid organs, such as the third eyelid and rectal mucosa. The major genotypes found on the flocks were ARQ/ARQ, ARQ/ARR, and ARQ/VRQ for codons 136, 154, and 171. Most of the sheep were considered to be at moderate to high risk, based on risk groups for developing scrapie. Some blood samples were sequenced, and polymorphisms were identified in other codons, such as 127, 142, and 143. Our data demonstrate the importance of preclinical scrapie diagnosis in Brazilian sheep, as most of the affected sheep showed no clinical signs, and emphasize the relevance of genotyping other Dorper sheep to determine the genotypic profile of the breed.
机译：Scrapie是一种影响绵羊和山羊的传染性神经变性疾病，与朊病毒蛋白的同种型的构象改变相关，导致宿主中枢神经系统中的沉积和聚集。自然疾病的发生可以受宿主遗传因子的影响，例如朊病毒蛋白基因的单个核苷酸多态性。本研究报告了三个受影响的Dorper群，位于巴西三个不同的农场。本研究的目的是使用Scrapie诊断分析这三个群，组织学，免疫组织化学，基因分型和Western印迹测定。对于免疫组织化学，选择了192只绵羊，并考虑了308个绵羊血液样品进行基因分型。通过免疫组织化学，共有22只绵羊呈污水阳性。其中，四种临床症状，并在蛋白质印迹测定中对OBEX进行了斯基稀抗性。没有临床迹象的绵羊在淋巴器官中是阳性的，例如第三个眼睑和直肠粘膜。羊群中发现的主要基因型是ARQ / ARQ，ARQ / ARR和ARQ / VRQ，用于密码子136,154和171.根据开发斯基费的风险群体，大多数绵羊被认为是中度至高风险。测序一些血液样品，在其他密码子中鉴定多态性，例如127,142和143.我们的数据表明了巴西绵羊临床前瘙痒病诊断的重要性，因为大多数受影响的绵羊都没有强调临床症状，并强调基因分型其他Dorper绵羊确定品种基因型剖面的相关性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号