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首页> 外文期刊>Tropical Animal Health and Production >Report of outbreaks of classical scrapie in Dorper sheep and associated prion protein gene polymorphisms in affected flocks
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Report of outbreaks of classical scrapie in Dorper sheep and associated prion protein gene polymorphisms in affected flocks

机译：关于多珀绵羊经典瘙痒病暴发和相关鸡群affected病毒蛋白基因多态性的报道

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Scrapie is an infectious neurodegenerative disease affecting sheep and goats, related with conformational alteration of an isoform of the prion protein that leads to deposition and aggregation in the host's central nervous system. Occurrence of the natural disease can be influenced by host genetic factors, such as a single nucleotide polymorphism of the prion protein gene. This study reports three scrapie-affected Dorper flocks located on three different farms in Brazil. The objective of this study was to analyze these three flocks using scrapie diagnostics, combining histology, immunohistochemistry, genotyping, and western blot assays. For immunohistochemistry, 192 sheep were selected and 308 sheep blood samples were taken for genotyping. A total of 22 sheep were scrapie positive by immunohistochemistry. Of these, four presented clinical signs and had scrapie immunoreactivity at the obex in western blot assays. The sheep without clinical signs were positive in lymphoid organs, such as the third eyelid and rectal mucosa. The major genotypes found on the flocks were ARQ/ARQ, ARQ/ARR, and ARQ/VRQ for codons 136, 154, and 171. Most of the sheep were considered to be at moderate to high risk, based on risk groups for developing scrapie. Some blood samples were sequenced, and polymorphisms were identified in other codons, such as 127, 142, and 143. Our data demonstrate the importance of preclinical scrapie diagnosis in Brazilian sheep, as most of the affected sheep showed no clinical signs, and emphasize the relevance of genotyping other Dorper sheep to determine the genotypic profile of the breed.
机译：瘙痒病是一种传染性神经退行性疾病，会影响绵羊和山羊，与the病毒蛋白同工型的构象改变有关，后者导致宿主中枢神经系统沉积和聚集。自然疾病的发生可能受到宿主遗传因素的影响，例如the病毒蛋白基因的单核苷酸多态性。这项研究报告了位于巴西三个不同农场的三个受瘙痒病影响的多珀鸡群。这项研究的目的是使用瘙痒病诊断方法，结合组织学，免疫组织化学，基因分型和蛋白质印迹分析方法，对这三个鸡群进行分析。为了进行免疫组织化学，选择了192只绵羊，并采集了308只绵羊血样进行基因分型。通过免疫组织化学，总共有22只绵羊为瘙痒病阳性。在这些蛋白中，有四个表现出临床体征，并且在western blot分析中在肥胖者处具有瘙痒病免疫反应性。没有临床体征的绵羊的淋巴器官，例如第三眼睑和直肠粘膜阳性。鸡群中发现的主要基因型分别是136、154和171位密码子的ARQ / ARQ，ARQ / ARR和ARQ / VRQ。根据形成瘙痒病的风险类别，大多数绵羊被认为处于中度到高风险。 。对一些血液样本进行了测序，并在其他密码子（例如127、142和143）中鉴定了多态性。我们的数据证明了在巴西绵羊中进行临床瘙痒病诊断的重要性，因为大多数受影响的绵羊没有临床体征，并强调对其他多珀绵羊进行基因分型的相关性，以确定该品种的基因型概况。

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