Investigating peste des petits ruminants (PPR) in naturally infected goats and sheep in Anseba Region, Eritrea, by reverse transcription polymerase chain reaction (RT-PCR)

Oshiek A. A.; Abdelkadir M. W.; Mihreteab Bereket; Mengesha S. B.; Teklay G. H.; Yemane H.; Mesfun H. G.; Efrem G. H.; Njeumi F.

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Investigating peste des petits ruminants (PPR) in naturally infected goats and sheep in Anseba Region, Eritrea, by reverse transcription polymerase chain reaction (RT-PCR)

机译：通过逆转录聚合酶链反应（RT-PCR）来调查天然感染的山羊和绵羊在天然感染的山羊和绵羊中的Peste des Petits反刍动物（PPR）

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The impact of peste des petits ruminants (PPR) virus was investigated by reverse transcription polymerase chain reaction (RT-PCR) on different samples obtained from non-vaccinated diseased and necropsied sheep and goats showing PPR-like symptoms. The disease picture was typical and sheep were observed to be less susceptible. Nasal and rectal swabs, whole blood and pathological tissue samples from the lungs, intestine, and mesenteric lymph nodes were used for this study. The results of RT-PCR indicated that from a total of 32 samples collected, 12 (41%) were positive by this method. Out of those collected samples, 29 were from goats and 3 were from sheep. Nasal and rectal swabs and blood samples were superior in detection of the PPR virus compared to other tissue samples.

机译：通过逆转录聚合酶链反应（RT-PCR）对从非接种疫苗的患病（RT-PCR）的不同样品进行研究，研究了Peste Des Petits反刍动物（PPR）病毒的影响，患有尸体绵羊和显示PPR样症状的山羊。疾病图片是典型的，并且观察到绵羊易感。鼻和直肠拭子，来自肺，肠和肠系膜淋巴结的全血和病理组织样品用于本研究。 RT-PCR的结果表明，通过收集的32个样品，通过该方法将12（41％）阳性。在这些收集的样品中，来自山羊和3来自羊。与其他组织样品相比，鼻和直肠拭子和血液样本在PPR病毒检测中优异。

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来源
《Tropical Animal Health and Production》 |2018年第4期|共6页
作者
Oshiek A. A.; Abdelkadir M. W.; Mihreteab Bereket; Mengesha S. B.; Teklay G. H.; Yemane H.; Mesfun H. G.; Efrem G. H.; Njeumi F.;
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作者单位

HAC Keren Eritrea;

HAC Keren Eritrea;

NAPHL Asmera Eritrea;

HAC Keren Eritrea;

HAC Keren Eritrea;

HAC Keren Eritrea;

HAC Keren Eritrea;

NAPHL Asmera Eritrea;

UN FAO Rome Italy;

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原文格式 PDF
正文语种 eng
中图分类畜牧、动物医学、狩猎、蚕、蜂;
关键词
Peste des petits ruminants virus; Reverse transcriptase polymerase chain reaction; Anseba Region; Eritrea;

机译：Peste des Petits反刍动物病毒;逆转录酶聚合酶链反应;Anseba地区;厄立特里亚;

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1. Investigating peste des petits ruminants (PPR) in naturally infected goats and sheep in Anseba Region, Eritrea, by reverse transcription polymerase chain reaction (RT-PCR) [J] . Oshiek A. A., Abdelkadir M. W., Mihreteab Bereket, Tropical Animal Health and Production . 2018,第4期

机译：通过逆转录聚合酶链反应（RT-PCR）来调查天然感染的山羊和绵羊在天然感染的山羊和绵羊中的Peste des Petits反刍动物（PPR）
2. Comparison of colorimetric loop-mediated isothermal amplification kit and reverse transcription-polymerase chain reaction in the diagnosis of peste des petits ruminants in sheep and goats in Southeast Nigeria [J] . Ijeoma Chekwube Chukwudi, Kenneth Ikejiofor Ogbu, Pam Dachung Luka, Veterinary World . 2020,第11期

机译：比色环介导的等温扩增试剂盒和逆转录聚合酶链反应在尼日利亚东南部绵羊和山羊Peste des Petits反刍动物诊断中的逆转录 - 聚合酶链反应
3. Sensitivity and specificity of real-time reverse transcription polymerase chain reaction, histopathology, and immunohistochemical labeling for the detection of Rift Valley fever virus in naturally infected cattle and sheep. [J] . Odendaal L., Fosgate G. T., Romito M., Journal of Veterinary Diagnostic Investigation . 2014,第1期

机译：实时逆转录聚合酶链反应，组织病理学和免疫组化标记法在自然感染牛和羊中检测裂谷热的敏感性和特异性。
4. Establishment of a duplex RT-PCR assay for simultaneous detection of Rift valley fever virus and peste des petits ruminants virus [C] . Li Guili, Wang Yin, Yao Xueping, International Conference on Materials, Environmental and Biological Engineering . 2015

机译：用于同时检测裂谷的双工RT-PCR测定和Peste des Petits反刍动物病毒的建立
5. Rapid detection of “Norwalk-like viruses” by reverse transcription-polymerase chain reaction (RT-PCR) and southern blot hybridisation (SBH) in outbreaks of acute gastroenteritis in eastern Ontario, Canada [D] . Wires, Shannon Meredith 2001

机译：通过逆转录聚合酶链反应（RT-PCR）和Southern blot杂交（SBH）快速检测加拿大安大略省东部急性肠胃炎暴发中的“ Norwalk样病毒”
6. Comparison of colorimetric loop-mediated isothermal amplification kit and reverse transcription-polymerase chain reaction in the diagnosis of peste des petits ruminants in sheep and goats in Southeast Nigeria [O] . Ijeoma Chekwube Chukwudi, Kenneth Ikejiofor Ogbu, Pam Dachung Luka, 2020

机译：比色环介导的等温扩增试剂盒和逆转录聚合酶链反应在尼日利亚东南部绵羊和山羊Peste des Petits反刍动物诊断中的逆转录 - 聚合酶链反应
7. Detection of Infectious Bursal Disease Virus (IBDV) in naturally infected chickens in Nigeria by the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) [O] . ES Haruna, DF Adene, VJ Gerrit 2005

机译：通过逆转录聚合酶链反应（RT-PCR）检测尼日利亚天然感染鸡的传染性Bursal疾病病毒（IBDV）

Investigating peste des petits ruminants (PPR) in naturally infected goats and sheep in Anseba Region, Eritrea, by reverse transcription polymerase chain reaction (RT-PCR)

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