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首页> 外文期刊>Tropical Animal Health and Production >High occurrence of CMY-2-type beta-lactamase-producing Escherichia coli among broiler flocks in Turkey
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High occurrence of CMY-2-type beta-lactamase-producing Escherichia coli among broiler flocks in Turkey

机译：在土耳其肉鸡羊群中产生高型CMY-2型β-内酰胺酶的大肠杆菌

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摘要

In this study, the prevalence of ESBL/pAmpC-producing Escherichia coli and their molecular characterization from cloacal swab samples were investigated. All samples were obtained from broiler flocks that are located in Hatay, Adana, and Mersin provinces of Turkey. Antimicrobial susceptibilities of the isolates were determined by disk diffusion method following the CLSI criteria. Genetic mechanisms mediating resistance in ESBL/pAmpC-producing E. coli isolates were identified by polymerase chain reaction (PCR) and followed by DNA sequencing. Phylogenetic groups and plasmid replicon types of the isolates were also investigated by PCR. The clonal relationship of selected isolates was investigated by enterobacterial repetitive intergenic consensus (ERIC)-PCR and multilocus sequence typing (MLST) method. Of 430 cloacal swab samples, 154 (35.8%) were positive for ESBL/pAmpC-producing E. coli. The ESBL/pAmpC type beta-lactamases were as follows: CMY-2 (n = 46), CMY-2 + TEM-1b (n = 63), SHV-12 (n = 5), SHV-12 + TEM-1b (n = 12), CTX-M-3 (n = 14), CTX-M-3 + TEM-1b (n = 1), CTX-M-15 (n = 4), CTX-M-15 + TEM-1b (n = 4), and CTX-M-1 (n = 3). Moreover, various rates of resistance to different antimicrobials were determined such as nalidixic acid (92.9%), ciprofloxacin (76%), sulfamethoxazole-trimethoprim (78.6%), tetracycline (73.4%), streptomycin (52.6%), chloramphenicol (44.2%), kanamycin (27.9%), tobramycin (24.7%), gentamicin (19.5%), and amikacin (0.6%). Furthermore, 148 (96.1%) isolates were found to be MDR. The ESBL/pAmpC-producing isolates were distributed into the following phylogroups: E (n = 61), B1 (n = 30), F (n = 20), A (n = 19), B2 (n = 11), D (n = 10), and C (n = 3). ERIC-PCR analysis showed 51 unrelated patterns. Out of the 28 selected isolates, the following sequence types (STs) were detected: ST354 (n = 3), ST114 (n = 3), ST5696 (n = 2), ST156 (n = 2), ST174 (n = 2), ST362 (n = 2), ST157 (n = 2), ST5114 (n = 2), ST6635, ST539, ST457, ST1640, ST95, ST5843, ST1158, ST10, ST648, and ST4248. The results of the current study revealed that broilers in Turkey are important reservoir of ESBL/pAmpC-producing E. coli, which suggest that these agents have a great potential of transmission to humans by food chain or direct contact.
机译：在该研究中，研究了ESBL / PAMPC产生的大肠杆菌的患病率及其从核糖拭子样品中的分子表征。所有样品均可从位于哈及，阿纳纳和土耳其的Mersin省份的肉鸡羊群中获得。通过CLSI标准之后的盘扩散法测定分离物的抗菌剂敏感性。通过聚合酶链式反应（PCR）鉴定了ESBL / PAMPC产生大肠杆菌分离物中抗抗抗抗ESBL / PAMPC的遗传机制，然后进行DNA测序。还通过PCR研究了系统发育基团和质粒复制子类型的分离物。通过肠杆菌重复性基因共识（ERIC）-PCR和多层序列键入（MLST）方法研究了所选分离株的克隆关系。在430个核糖棉状样品中，154（35.8％）为ESBL / PAMPC生产大肠杆菌阳性。 ESBL / PAMPC型β-内酰胺酶如下：CMY-2（n = 46），CMY-2 + TEM-1B（n = 63），SHV-12（n = 5），SHV-12 + TEM-1B （n = 12），CTX-M-3（n = 14），CTX-M-3 + TEM-1B（n = 1），CTX-M-15（n = 4），CTX-M-15 + TEM -1b（n = 4）和ctx-m-1（n = 3）。此外，确定了不同抗微生物的各种抗性抗性耐药率（92.9％），环丙沙星（76％），磺胺甲恶唑 - 三甲双胍（78.6％），四环素（73.4％），链霉素（52.6％），氯霉素（44.2％） ），卡那霉素（27.9％），染发蛋白（24.7％），庆大霉素（19.5％）和Amikacin（0.6％）。此外，发现148例（96.1％）分离株是MDR。将ESBL / PAMPC产生分离物分布到以下晶粒中：E（n = 61），b1（n = 30），f（n = 20），a（n = 19），b2（n = 11），d （n = 10），和c（n = 3）。 ERIC-PCR分析显示了51个无关的模式。除了28个选定的分离物中，检测到以下序列类型（STS）：ST354（n = 3），ST114（n = 3），ST5696（n = 2），ST156（n = 2），ST174（n = 2 ），ST362（n = 2），ST157（n = 2），ST5114（n = 2），ST6635，ST539，ST457，ST1640，ST95，ST5843，ST1158，ST10，ST648和ST4248。目前的研究结果表明，土耳其的肉鸡是ESBL / PAMPC生产大肠杆菌的重要储层，表明这些试剂通过食物链或直接接触具有对人类传播的巨大潜力。

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