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首页> 外文期刊>Veterinary Parasitology >Comparative analysis of the Leishmania infantum-specific antibody repertoires and the autoantibody repertoires between asymptomatic and symptomatic dogs
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Comparative analysis of the Leishmania infantum-specific antibody repertoires and the autoantibody repertoires between asymptomatic and symptomatic dogs

机译：对嗜血管幼儿特异性抗体曲目的比较分析和无症状和症状犬之间的自身抗体曲目

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摘要

Leishmania (L.) infantum-infected dogs may present with a large range of clinical signs, from apparently healthy with no or few (asymptomatic dogs, AD) to several clinical signs indicators of active infection (symptomatic dogs, SD). The present study is justified by the conflicting reports describing that either L. infantum-specific IgGI or IgG2 antibodies may be used as isotype marker of the asymptomatic infection status and by the lack, to our knowledge, of previous analysis of the IgG sub-classes autoantibody repertoires of Leishmania-infected dogs. On the basis of clinical evaluation and laboratory testing (IFAT, parasitological examination of Giemsa-stained lymph node smears, L. infantum antigens-ELISA of total (Tot) IgG), 131 dogs were categorized as SD, asymptomatic seronegative (AND) or seropositive dogs (APD) from surrounding areas, and as negative control dogs (CTD). ELISA based on leishmanial native antigens or recombinant LACK and LeIF proteins showed that SD produce higher levels of specific Tot IgG, IgGI and IgG2 antibodies than APD, and that for both clinical stages, the antibody titers of IgG2 isotype were constantly higher than those of the IgGI. The seroprevalences of Tot IgG, IgG2 did not differ between APD and SD groups (97 and 97% in SD; 100 and 96% in APD, respectively) whereas that of IgGI was slightly lower in SD (88% of APD versus 82% of SD). The autoantibody repertoires were analyzed by ELISA using HEp-2 extracts, ds-DNA, human albumin and transferrin as self-antigens and by Western blot using HEp-2 proteins. ELISA results' indicated that APD develop higher levels of IgGI auto antibodies, and higher seroprevalence (50% and 26% in APD and SD, respectively), contrasting with lower levels and seroprevalences of Tot IgG and IgG2 (43 and 68% for APD; 100 and 74% for SD). Interestingly, SD showed a stronger IgGI and particularly IgG2 reactivity with transferrin, an iron-binding protein, than APD and AND. Western blotting experiments produced heterogeneous IgGI and IgG2 inter- and intra-groups reactivity profiles towards HEp-2 proteins, to identify a specific antigenic profile. Generated data from competitive HEp-2-ELISA using leishmanial antigens as inhibitors were in favor that IgGI antibodies are predominantly autoantibodies to self-antigens in APD whereas they are mainly cross-reactive (Leishmania/self-antigens) in SD.
机译：Leishmania（L.）感染的狗可能出现大量的临床症状，从显然健康，没有或少数（无症状狗，AD）到几个活性感染的临床标志指标（症状狗，SD）。本研究由描述L.Igantum特异性IgGI或IgG2抗体的矛盾的报告是合理的，所述IGG2抗体可以用作无症状感染状况的同种型标志物，并且通过缺乏对IGG子类的缺乏分析的缺乏分析自身抗体感染狗的曲目。在临床评价和实验室检测的基础上（IFAT，Giemsa染色的淋巴结涂抹术，L.Igantum抗原-ELISA）总量（Tot）IgG），131只狗被分类为SD，无症状血清可（和）或血清阳性来自周围区域的狗（APD），以及阴性对照犬（CTD）。基于LeishManial Native抗原或重组缺乏和Leif蛋白的ELISA显示SD产生更高水平的特定Tot IgG，IgGI和IgG2抗体，而对于临床阶段，IgG2同种型的抗体滴度始终高于那些Iggi。 Tot IgG的Seroprevalences，IgG2在APD和SD基团之间没有区别（SD中97和97％，分别在APD中的96％和96％），而IgGi的SD略低于略低（88％的APD与82％的APD SD）。通过ELISA使用HEP-2提取物，DS-DNA，人白蛋白和转移素作为自我抗原和使用HEP-2蛋白的蛋白质印迹进行分析自身抗体曲目。 ELISA结果表明，APD发育较高水平的IgGI自动抗体，较高的SEROPREVALING（分别为APD和SD的50％和26％），与TOT IgG和IgG2的较低水平和SEROPREVALINGS对比（43和78％的APD; 100和74％的SD）。有趣的是，SD显示出具有较强的IgGI，特别是IgG2与转铁蛋白，铁结合蛋白的反应性，而不是APD和。 Western印迹实验产生非均相IgGI和IgG2朝向Hep-2蛋白的基团间和内反应性谱，以鉴定特定的抗原性剖面。使用LeishManial抗原作为抑制剂的竞争性HEP-2-ELISA产生的数据有利于IgGI抗体主要在APD中的自我抗原自身抗原，而它们主要是SD中的交叉反应性（Leishmania / Self-antigens）。

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