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首页> 外文期刊>Veterinary Parasitology >Expression and serodiagnostic potential of antigen B and thioredoxin peroxidase from Taenia multiceps
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Expression and serodiagnostic potential of antigen B and thioredoxin peroxidase from Taenia multiceps

机译：塔尼亚Multi上浆抗原B和Thiroedoxin过氧化物酶的表达和血清诊断潜力

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摘要

Coenurosis is a serious parasitic disease of herbivorous animals caused by the metacestode of Taenia multiceps (Coenurus cerebralis). Accordingly, a significant amount of research is currently dedicated to the development of appropriate antigens for use in rapid and accurate coenurosis diagnosis kits. In the present study, antigen B (AgB) and thioredoxin peroxidase (TPx) from T. multiceps were cloned and expressed using a prokaryotic system, molecular characterization of Tm-AgB was determined by bioinformatical analyses. The serological diagnostic potentials of rTm-AgB and rTm-TPx were evaluated by indirect ELISA and compared with those of previously reported rTm-AnxB2, rTm-HSP70, and rTm-GST. The results showed that Tm-AgB is a specific lipoprotein of cestodes with good thermal stability. The ELISA assay showed that rTm-AgB exhibited a sensitivity of 95.8% and a specificity of 87.5%, indicating its strong potential for serological diagnosis of T. multiceps.
机译：长病是由Taenia Mutericps（Coenurus Cerebralis）的MetaceTode引起的食草动物的严重寄生疾病。 因此，目前致力于开发适当的抗原用于快速和准确的长期尿精诊断套件的研究。 在本研究中，使用原核系统克隆并表达来自T.多样的抗原B（AGB）和硫氧嗪过氧化物酶（TPX），通过生物信息分析测定TM-AGB的分子表征。 通过间接ELISA评估RTM-AGB和RTM-TPX的血清学诊断电位，并与先前报道的RTM-ANXB2，RTM-HSP70和RTM-GST进行比较。 结果表明，TM-AGB是具有良好热稳定性的Cestod的特定脂蛋白。 ELISA测定表明，RTM-AGB表现出95.8％的敏感性，特异性为87.5％，表明其血清学诊断的强大潜力。

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