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Effect of the Length of the Cycle on Biodegradable Polymer Production and Microbial Community Selection in a Sequencing Batch Reactor

机译:循环长度对测序间歇反应器中可生物降解聚合物生产和微生物群落选择的影响

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The effect of the length of the cycle on the enrichment and selection of mixed cultures in sequencing batch reactors(SBRs)has been studied,with the aim of biodegradable polymers(namely,polyhydroxyalkanoates(PHAs))production from organic wastes.At a fixed feed concentration(20 gCOD/L)and organic loading rate(20 gCOD/L/day),the SBR was operated at different lengths of the cycle,in the range 1-8 h.Process performance was measured by considering the rates and yields of polymer storage and of the competing phenomenon of growth.The selected biomass was enriched with microorganisms that were able to store PHAs at high rates and yields only when the length of the cycle was 2 or 4 h,even though in these conditions the process was unstable.On the other hand,when the length of the cycle was 1 or 8 h,the dynamic response of the selected microorganisms was dominated by growth.The best process performance was characterized by storage rates in the range 500-600 mgCOD/gCOD/h and storage yields of 0.45-0.55 COD/COD.The corresponding productivity of the process was in the range 0.25-0.30 gPHA/L/h,the highest values obtained until now for mixed cultures.The microbial composition of the selected biomasses was analyzed through denaturing gradient gel electrophoresis(DGGE)and reverse-transcriptase denaturing gradient gel electrophoresis(RT-DGGE).The instability of the runs characterized by high storage rate was associated with a higher microbial heterogeneity compared to the runs with a stable growth response.
机译:研究了循环长度对顺序分批反应器(SBR)中混合培养物富集和选择的影响,目的是从有机废物中生产可生物降解的聚合物(即聚羟基链烷酸酯(PHA))。浓度(20 gCOD / L /天)和有机负载量(20 gCOD / L /天)下,SBR在不同的循环周期内操作,持续时间为1-8 h。选定的生物量富含微生物,这些微生物能够以较高的速率存储PHA,并且仅在周期长度为2或4 h时才产生产量,即使在这些条件下过程也是不稳定的另一方面,当周期长度为1或8 h时,所选微生物的动态响应受生长支配。最佳工艺性能的特征在于其储存速率为500-600 mgCOD / gCOD / h和存储产量为0.45 -0.55 COD / COD。该过程的相应生产率在0.25-0.30 gPHA / L / h的范围内,这是迄今为止混合培养所获得的最高值。通过变性梯度凝胶电泳分析所选生物质的微生物组成( DGGE)和逆转录酶变性梯度凝胶电泳(RT-DGGE)。与具有稳定生长响应的运行相比,以高存储速率为特征的运行不稳定性与较高的微生物异质性有关。

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