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首页> 外文期刊>Veterinary Research Communications >THE USE OF CULTURED HEPATOCYTES FROM GOATS AND CATTLE TO INVESTIGATE XENOBIOTIC OXIDATIVE METABOLISM
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THE USE OF CULTURED HEPATOCYTES FROM GOATS AND CATTLE TO INVESTIGATE XENOBIOTIC OXIDATIVE METABOLISM

机译：从山羊和牛的培养肝细胞使用培养肝细胞来研究异卵氧化代谢

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摘要

The oxidative metabolism of aldicarb (ALD), a carbamate pesticide, and fenbendazole (FBZ), an anthelmintic, was studied using cultured hepatocytes obtained from 4 goats and a bullock and incubated with ALD (50 mu mol/L) and FBZ (10 mu mol/L). The parent compounds and the metabolites were measured by HPLC. Both compounds are metabolized at the sulphur atom via two sequential oxidations, first to the sulphoxide (aldicarb sulphoxide and oxfendazole, respectively) and then to the sulphone. Oxfendazole and fenbendazole sulphone from FBZ, and aldicarb sulphoxide from ALD were found in both species. Aldicarb sulphone was not produced by the hepatocyte preparations from the bullock. The good correlation obtained comparing the in vitro results of FBZ metabolism with published in vivo data obtained on FBZ kinetics in the same species confirmed the usefulness of in vitro models for predictive analysis of in vivo xenobiotic biotransformations.
机译：使用从4只山羊和牛的培养的肝细胞研究了醛癌（ALD），氨基甲酸酯农药和Fenbendazole（FBZ），氨基甲酰杀虫剂和Fenbendazole（FBZ），并与ALD（50μmol/ L）和FBZ孵育（10μm mol / l）。 通过HPLC测量母体化合物和代谢物。 两种化合物通过两个顺序氧化在硫原子中代谢，首先至硫氧化物（分别分别为硫氧化铵和氧杂唑），然后加入砜。 来自FBZ的牛津唑和Fenbendazole Sulphone，并在两种物种中发现来自ALD的醛酰亚胺。 肝细胞从肝细胞制剂中没有生产的醛癌砜。 在同一物种中获得的FBZ动力学中获得的体内数据中发表的FBZ代谢的体外结果进行了比较的良好相关性证实了体外模型对体内异丙菌生物转化的预测分析的有用性。

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