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首页> 外文期刊>Biotechnology Progress >Enhanced Vanillin Production from Recombinant E.coli Using NTG Mutagenesis and Adsorbent Resin
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Enhanced Vanillin Production from Recombinant E.coli Using NTG Mutagenesis and Adsorbent Resin

机译：使用NTG诱变和吸附树脂增强重组大肠杆菌生产香兰素

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摘要

Vanillin production was tested with different concentrations of added ferulic acid in E.coli harboring plasmid pTAHEF containing fcs(feruloyl-CoA synthase)and ech(enoyl-CoA hydratase/aldolase)genes cloned from Amycolatopsis sp.strain HR104.The maximum production of vanillin from E.coli DH5a harboring pTAHEF was found to be 1.0 g/L at 2.0 g/L of ferulic acid for 48 h of culture.To improve the vanillin production by reducing its toxicity,two approaches were followed:(1)generation of vanillin-resistant mutant of NTG-VR1 through NTG mutagenesis and(2)removal of toxic vanillin from the medium by XAD-2 resin absorption.The vanillin production of NTG-VR1 increased to three times at 5 g/L of ferulic acid when compared with its wild-type strain.When 50%(w/v)of XAD-2 resin was employed in culture with 10 g/L of ferulic acid,the vanillin production of NTG-VR1 was 2.9 g/L,which was 2-fold higher than that obtained with no use of the resin.
机译：在含有质粒pTAHEF的大肠杆菌中以不同浓度添加的阿魏酸对香兰素的生产进行了测试，该质粒包含从淀粉样芽孢杆菌HR104菌株中克隆的fcs（阿魏酰辅酶A合酶）和ech（烯酰辅酶A水合酶/醛缩酶）基因的克隆。在48 h的培养中，发现含有pTAHEF的大肠杆菌DH5a在2.0 g / L的阿魏酸下为1.0 g / L。为了通过降低其毒性来提高香兰素的产量，遵循以下两种方法：（1）香兰素的产生NTG诱变和（2）通过XAD-2树脂吸收从培养基中去除有毒香兰素的耐药性突变体。在5 g / L阿魏酸下，NTG-VR1的香兰素产量增加了三倍当将50％（w / v）的XAD-2树脂与10 g / L的阿魏酸一起培养时，NTG-VR1的香兰素产量为2.9 g / L，是原来的2倍高于不使用树脂获得的树脂。

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