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首页> 外文期刊>Vlaams Diergeneeskundig Tijdschrift >A comparative study of techniques used for the diagnosis of effusive feline infectious peritonitis
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A comparative study of techniques used for the diagnosis of effusive feline infectious peritonitis

机译：用于诊断活性猫畜腹膜炎的技术的比较研究

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摘要

Feline infectious peritonitis (FIP) is a fatal disease caused by feline infectious peritonitis virus (FIPV). At present, neither a licensed treatment nor an accurate ante-mortem diagnosis are available. In the present study, three available tests were evaluated for their diagnostic power on effusion samples. High feline coronavirus antibody titers, measured with an immunoperoxidase monolayer assay (IPMA), were correlated with FIP but its low specificity precluded a reliable diagnosis. The in-house 5' reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) provided a much better specificity and high sensitivity. Given the low sensitivity of immunofluorescence staining (IF) of effusive cells, the RT-qPCR alone or in combination with IPMA represents a good alternative for IF. In the majority of the effusion samples from FIP positive animals, Sanger sequencing of the open reading frame encoding the spike protein (ORF S) revealed not only mutations that were previously associated with FIP (M1058L, S1060A, 11106T and D1108Y/E/G) but also two new, closely related mutations (T1112S/N).
机译：猫培养的传染性腹膜炎（FIP）是由猫传染性腹膜炎病毒（FIPV）引起的致命疾病。目前，持牌治疗既没有可用许可的治疗，也没有准确的抗诊断诊断。在本研究中，评估了三种可用的测试，用于它们对积液样品的诊断功能。用免疫氧化酯酶单层测定（IPMA）测量的高猫冠状病毒抗体滴度与FIP相关，但其低特异性排除了可靠的诊断。内部5'逆转录酶定量聚合酶链反应（RT-QPCR）提供了更好的特异性和高灵敏度。考虑到散滤染色的低灵敏度（IF）的活性细胞，单独的RT-QPCR或与IPMA的组合代表了良好的替代方案。在来自FIP阳性动物的大部分积液样品中，编码尖峰蛋白（ORF S）的开放阅读框的Sanger测序揭示了先前与FIP（M1058L，S1060A，11106T和D1108Y / E / G）相关的突变而且还有两个新的，密切相关的突变（T1112S / N）。

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