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Collection and cryopreservation of epididymal spermatozoa in dogs

机译:狗附睾精子的收集和冷冻保存

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摘要

When a male dog is not able to ejaculate, epididymal spermatozoa can be used to artificially inseminate the bitch. In the present study, two methods to collect epididymal sperm after castration were compared, i.e. the float-up method and the aspiration method. With the float-up method, more spermatozoa were collected than with the aspiration method (1810 +/- 718.1 million versus 694 +/- 244.5 million respectively, p-value = 0.018). A trend towards a higher post-thaw motility was observed when using the float-up method compared to the aspiration method (52 +/- 9.4% versus 44 +/- 9.4%, p-value = 0.060). Semen samples collected with the float-up method showed more blood contamination than with the aspiration method; however, this seemed to have no effect on the quality and quantity of the semen. It can therefore be concluded that the float-up method is the method of choice when epididymal sperm has to be collected in dogs.
机译:当雄性狗不能射精时,附睾精子可用于人工体制婊子。 在本研究中,比较阉割后收集附睾精子的两种方法,即浮动方法和抽吸方法。 通过浮动方法,收集更多的精子,而不是吸气法(1810 +/- 718.1百万与694 +/- 244.5百万,p值= 0.018)。 与吸入方法相比,使用浮动方法(52 +/- 9.4%,对44 +/- 9.4%,P值= 0.060)时,观察到浮动后促进后动力的趋势。 用浮动方法收集的精液样品显示比抽吸方法更多的血液污染; 然而,这似乎对精液的质量和数量没有影响。 因此,可以得出结论,浮动方法是当必须在狗中收集附睾精子时的选择方法。

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    Univ Ghent Fac Diergeneeskunde Vakgroep Verloskunde Voortplanting &

    Bedrijfsdier Salisburylaan 133 B-9820 Merelbeke Belgium;

    Univ Ghent Fac Diergeneeskunde Vakgroep Verloskunde Voortplanting &

    Bedrijfsdier Salisburylaan 133 B-9820 Merelbeke Belgium;

    Univ Ghent Fac Diergeneeskunde Vakgroep Verloskunde Voortplanting &

    Bedrijfsdier Salisburylaan 133 B-9820 Merelbeke Belgium;

    Univ Ghent Fac Diergeneeskunde Vakgroep Verloskunde Voortplanting &

    Bedrijfsdier Salisburylaan 133 B-9820 Merelbeke Belgium;

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  • 正文语种 dut
  • 中图分类 动物医学(兽医学);
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