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首页> 外文期刊>Biotechnology Progress >Influence of Peptide Ligand Surface Density and Ethylene Oxide Spacer Arm on the Capture of Porcine Parvovirus
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Influence of Peptide Ligand Surface Density and Ethylene Oxide Spacer Arm on the Capture of Porcine Parvovirus

机译：肽配体表面密度和环氧乙烷间隔臂对猪细小病毒捕获的影响

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摘要

In previous work, we identified two trimeric peptide ligands (designated WRW and KYY), which bound specifically to porcine parvovirus (PPV) and demonstrated their ability to capture and remove the virus from solutions containing 7.5% human blood plasma. This article examines the influences of peptide density and the presence of an ethylene oxide spacer arm on the efficiency of virus capture using these two ligands. The WRW peptide bound the most virus from plasma solutions at the lowest peptide density tested (0.008 mmollg dry resin), and binding was enhanced by the presence of the spacer arm. On the other hand, the KYY peptide bound the most viruses at the same low peptide density, but it performed better in the absence of the spacer arm. Of the two, the binding efficiency of the WRW peptide was more sensitive to peptide density and spacer arm presence. These results indicate that low peptide densities enhance binding selectivity, facilitating specific peptide-virus binding even in the presence of plasma proteins which can theoretically bind nonspecifically.
机译：在先前的工作中，我们鉴定了两个三聚体肽配体（分别称为WRW和KYY），它们与猪细小病毒（PPV）特异性结合，并证明了它们能够从含有7.5％人体血浆的溶液中捕获和去除病毒。本文研究了肽密度和环氧乙烷间隔臂的存在对使用这两个配体的病毒捕获效率的影响。 WRW肽以测试的最低肽密度（0.008 mmol / g干树脂）结合了血浆溶液中的最多病毒，并且通过间隔臂的存在增强了结合。另一方面，KYY肽以相同的低肽密度结合大多数病毒，但在没有间隔臂的情况下表现更好。在这两者中，WRW肽的结合效率对肽密度和间隔臂的存在更为敏感。这些结果表明，低的肽密度提高了结合选择性，即使在存在理论上可以非特异性结合的血浆蛋白的情况下，也促进了特定的肽-病毒结合。

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