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首页> 外文期刊>The International journal of oral & maxillofacial implants >The Effect of Dissociated Soft Tissue on Osteogenesis: A Preliminary In Vitro Study
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The Effect of Dissociated Soft Tissue on Osteogenesis: A Preliminary In Vitro Study

机译：解离软组织对成骨的影响：初步体外研究

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Purpose: The purpose of this in vitro study was to examine the effect of dissociated soft tissue on bone marrow cell proliferation and differentiation under osteogenic conditions. Materials and Methods: Rat bone marrow ceils were cultured to assess the stimulation of cell proliferation and differentiation. Harvested palatal mucosa was dissociated using a device (Rigenera, Human Brain Wave), and the dissociated soft tissue was cultured with rat bone marrow cells. Cell proliferation, differentiation, and mineralized nodule formation were assessed after 2 or 5 days of culturing. Bone marrow cell proliferation was assessed by quantifying the absorbance of a water-soluble tetrazolium salt using a cell proliferation assay kit. Bone marrow cell differentiation was assessed by alkaline phosphatase staining and real-time polymerase chain reaction. Mineralized nodule formation was assessed by Alizarin red staining. Results: At day 2, cell proliferation, osteoblast-specific gene expression, and mineralized nodule formation were significantly higher in the experimental group than in the control group. Alkaline phosphatase staining was also higher in the experimental group on day 2. Mineralized nodule formation area and osteoblast-specific gene expression were also statistically higher in the experimental group on day 5. Conclusion: This study demonstrates that dissociated soft tissue elevates bone marrow cell proliferation and differentiation under osteogenic conditions.
机译：目的：这种体外研究的目的是检查解离软组织对骨髓细胞增殖和骨质骨质条件下分化的影响。材料和方法：培养大鼠骨髓细胞以评估细胞增殖和分化的刺激。使用器件（RigEnera，人脑波）解离收获的腭粘膜，并用大鼠骨髓细胞培养解离软组织。在培养2或5天后评估细胞增殖，分化和矿化结节形成。通过使用细胞增殖测定试剂盒量化水溶性四唑鎓盐的吸光度来评估骨髓细胞增殖。通过碱性磷酸酶染色和实时聚合酶链反应评估骨髓细胞分化。通过茜素红染色评估矿化结节形成。结果：实验组在第2天，细胞增殖，特异性特异性基因表达和矿化结节形成显着高于对照组。在第2天的实验组中碱性磷酸酶染色也较高。在第5天的实验组中，矿化结节形成区域和成骨细胞特异性基因表达也在统计学上更高。结论：本研究表明解离软组织升高了骨髓细胞增殖骨质发生条件下的分化。

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