• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Feed Development for Fed-Batch CHO Production Process by Semisteady State Analysis
【24h】

Feed Development for Fed-Batch CHO Production Process by Semisteady State Analysis

机译：半稳态分析法生产补料分批CHO生产工艺的饲料

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Semisteady state cultures are useful for studying cell physiology and facilitating media development. Two semisteady states with a viable cell density of 5.5 million cellslmL were obtained in CHO cell cultures and compared with a fed-batch mode control. In the first semisteady state, the culture was maintained at 5 mM glucose and 0.5 mM glutamine. The second condition had threefold higher concentrations of both nutrients, which led to a 10% increase in lactate production, a 78% increase in ammonia production, and a 30% reduction in cell growth rate. The differences between the two semisteady states indicate that maintaining relatively low levels of glucose and glutamine can reduce the production of lactate and ammonia. Specific amino acid production and consumption indicated further metabolic differences between the two semisteady states and fed-batch mode. The results from this experiment shed, light in the feeding strategy for a fed-batch process and feed medium enhancement. The fed-batch process utilizes a feeding strategy whereby the feed added was based on glucose levels in the bioreactor. To evaluate if a fixed feed strategy would improve robustness and process consistency, two alternative feeding strategies were implemented. A constant volume feed of 30% or 40% of the initial culture volume fed over the course of cell culture was evaluated. The results indicate that a constant volumetric-based feed can be more beneficial than a glucose-based feeding strategy. This study demonstrated the applicability of analyzing CHO cultures in semisteady state for feed enhancement and continuous process improvement.
机译：半稳态状态培养对于研究细胞生理学和促进培养基发育非常有用。在CHO细胞培养中获得了两个半稳态，其活细胞密度为550万个细胞/ mL，并与分批补料模式对照进行了比较。在第一半稳态下，将培养物维持在5 mM葡萄糖和0.5 mM谷氨酰胺下。第二种条件是两种营养素的浓度都增加了三倍，这导致乳酸产量增加了10％，氨气产量增加了78％，细胞生长速度降低了30％。两种半稳态之间的差异表明，维持相对较低的葡萄糖和谷氨酰胺水平可以减少乳酸和氨的产生。特定氨基酸的产生和消耗表明两个半稳态和补料分批模式之间的新陈代谢进一步不同。该实验的结果揭示了补料分批进料和进料介质增强的进料策略。补料分批工艺利用补料策略，由此添加的补料基于生物反应器中的葡萄糖水平。为了评估固定进料策略是否会提高鲁棒性和过程一致性，实施了两种替代进料策略。评估了在细胞培养过程中进料的初始培养物体积的30％或40％的恒定体积进料。结果表明，恒定的基于体积的饲料比基于葡萄糖的喂养策略更有益。这项研究证明了分析半稳定状态的CHO培养物对饲料增强和连续过程改进的适用性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号