• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Single Step Purification of Lactoperoxidase from Whey Involving Reverse Micelles-Assisted Extraction and its Comparison with Reverse Micellar Extraction
【24h】

Single Step Purification of Lactoperoxidase from Whey Involving Reverse Micelles-Assisted Extraction and its Comparison with Reverse Micellar Extraction

机译：乳清中涉及反胶束辅助萃取的乳过氧化物酶的一步纯化及其与反胶束萃取的比较

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The extraction of lactoperoxidase (EC 1.11.1.7) from whey was studied using single step reverse micelles-assisted extraction and compared with reverse micellar extraction. The reverse micelles-assisted extraction resulted in extraction of contaminating proteins and recovery of lactoperoxidase in the aqueous phase leading to its purification. Reverse micellar extraction at the optimized condition after forward and backward steps resulted in activity recovery of lactoperoxidase and purification factor of the order of 86.60% and 3.25-fold, respectively. Whereas reverse micelles-assisted extraction resulted in higher activity recovery of lactoperoxidase (127.35%) and purification factor (3.39-fold). The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) profiles also evidenced that higher purification was obtained in reverse micelles-assisted extraction as compared of reverse micellar extracted lactoperoxidase.
机译：使用单步反胶束辅助萃取研究了乳清中乳过氧化物酶（EC 1.11.1.7）的萃取，并与反胶束萃取进行了比较。反胶束辅助萃取导致污染蛋白的萃取和水相中乳过氧化物酶的回收，从而导致其纯化。在向前和向后步骤后，在最佳条件下进行逆胶束萃取可分别恢复乳过氧化物酶的活性和纯化因子，分别达到86.60％和3.25倍。而反胶束辅助提取可提高乳过氧化物酶的活性回收率（127.35％）和纯化因子（3.39倍）。十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）谱也证明，与反胶束萃取的乳过氧化物酶相比，在反胶束辅助萃取中获得了更高的纯度。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号