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首页> 外文期刊>Biotechnology Progress >Fast Enzymatic Saccharification of Switchgrass After Pretreatment with Ionic Liquids
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Fast Enzymatic Saccharification of Switchgrass After Pretreatment with Ionic Liquids

机译：离子液体预处理后的柳枝快速酶促糖化

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The pretreatment of cellulose using ionic liquids (ILs) has been shown to be an effective method for improving the enzymatic hydrolysis of cellulose; this technique affords a fast and complete saccharification of cellulose into reducing sugars (Dadi et al., Biotechnol Bioeng. 2006; 95:904-910; Liu and Chen, Chinese Sci Bull. 2006; 51:2432-2436; Zhao et al, J Biotechnol. 2009; 139:47-54). Motivated by these advances, this study examines the effect of IL-pretreatment on the enzymatic hydrolysis of purified xylan (as a model system of hemi-cellulose) and switchgrass (as a real lignocellulose). The IL-pretreatment resulted in no improvement in the hydrolysis of xylan. The likely reason is that pure xylan has a low degree of polymerization (DP), and is readily biodegraded even without any pretreatment. However, in real cellulosic materials (such as switchgrass), xylan is entrapped within the cellulosic matrix, and cannot be conveniently accessed by enzymes. Our data demonstrate that the IL-pretreatment of switchgrass significantly improved the enzymatic saccharification of both cellulose (96% D-glucose yield in 24 h) and xylan (63% D-xylose yield in 24 h). The compositional analysis of switchgrass suggests a lower lignin content after IL-pretreatment. In addition, the infrared spectrum of regenerated switchgrass indicates a lower substrate crystallinity, whereas the enzyme adsorption isotherm further implies that the regenerated substrate is more accessible to enzymes. This study has further confirmed that IL-pretreatment is an effective tool in enhancing the enzymatic hydrolysis of cellulosic biomass, and allowing a more complete saccharification.
机译：使用离子液体（ILs）预处理纤维素已被证明是改善纤维素酶促水解的有效方法。这项技术可将纤维素快速而完整地糖化为还原糖（Dadi等，Biotechnol Bioeng。2006; 95：904-910; Liu and Chen，Chinese Sci Bull。2006; 51：2432-2436; Zhao等， J Biotechnol。2009; 139：47-54）。受这些进展的推动，本研究研究了IL预处理对纯化的木聚糖（作为半纤维素的模型系统）和柳枝（（作为真正的木质纤维素）的酶促水解的影响。 IL预处理未导致木聚糖水解的改善。可能的原因是纯木聚糖的聚合度（DP）较低，即使不进行任何预处理也很容易被生物降解。但是，在真正的纤维素材料（如柳枝switch）中，木聚糖被困在纤维素基质中，不能被酶方便地进入。我们的数据表明，柳枝IL的IL预处理显着改善了纤维素（24小时内96％D-葡萄糖产率）和木聚糖（24小时内63％D-木糖产率）的酶促糖化作用。柳枝switch的成分分析表明，IL预处理后木质素含量较低。另外，再生柳枝infrared的红外光谱表明较低的底物结晶度，而酶吸附等温线进一步暗示了再生底物更容易被酶接近。这项研究进一步证实，IL预处理是增强纤维素生物质酶促水解并实现更完全糖化的有效工具。

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