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首页> 外文期刊>Biotechnology Progress >Probing Protein Colloidal Behavior in Membrane-Based Separation Processes Using Spectrofluorometric Rayleigh Scattering Data
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Probing Protein Colloidal Behavior in Membrane-Based Separation Processes Using Spectrofluorometric Rayleigh Scattering Data

机译：使用分光荧光瑞利散射数据探查基于膜的分离过程中的蛋白质胶体行为

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One of the primary problems in membrane-based protein separation is membrane fouling. In this study we explored the feasibility of employing Rayleigh light scattering data from fluorescence studies combined with chemometric techniques to determine whether a correlation could be established with membrane fouling phenomena. Membrane flux was measured in a dead-end UF filtration system and the effect of protein solution properties on the flux decline was systematically investigated. A variety of proteins were used as a test case in this study. In parallel, the colloidal behavior of the protein solutions was assessed by employing multi-wavelength Rayleigh scattering measurements. To assess the usefulness of Rayleigh scattering measurements for probing the colloidal behavior of proteins, a protein solution of β-lactoglobulin was used as a base-case scenario. The colloidal behavior of different β-lac-toglobulin solutions was inferred based on published data for this protein, under identical solution conditions, where techniques other than Rayleigh scattering had been used. Using this approach, good agreement was observed between scattering data and the colloidal behavior of this protein. To test the hypothesis that a high degree of aggregation will lead to increased membrane fouling, filtration data was used to find whether the Rayleigh scattering intensity correlated with permeate flux changes. It was found that for protein solutions which were stable and did not aggregate, fouling was reduced and these solutions exhibited reduced Rayleigh scattering. When the aggregation behavior of the solution was favored, significant flux declines occurred and were highly correlated with increased Rayleigh scattering.
机译：基于膜的蛋白质分离的主要问题之一是膜污染。在这项研究中，我们探索了将来自荧光研究的瑞利光散射数据与化学计量学技术结合使用以确定是否可以与膜污染现象建立相关性的可行性。在无端超滤系统中测量膜通量，并系统研究蛋白质溶液性质对通量下降的影响。在这项研究中，多种蛋白质被用作测试案例。同时，通过采用多波长瑞利散射测量来评估蛋白质溶液的胶体行为。为了评估瑞利散射测量对探测蛋白质胶体行为的有用性，将β-乳球蛋白的蛋白质溶液用作基本案例。根据已发布的该蛋白质的数据，在相同的溶液条件下（使用瑞利散射技术以外的其他技术），基于该蛋白质的不同数据推断出不同的β-lac-球蛋白溶液的胶体行为。使用这种方法，在散射数据和该蛋白的胶体行为之间观察到良好的一致性。为了检验高度聚集会导致膜污染增加的假说，使用过滤数据来查找瑞利散射强度是否与渗透通量变化相关。发现对于稳定且不聚集的蛋白质溶液，结垢减少，并且这些溶液表现出减少的瑞利散射。当有利于溶液的聚集行为时，通量会显着下降，并且与瑞利散射的增加高度相关。

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