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首页> 外文期刊>Biotechnology Progress >Reverse Micelles-Mediated Transport of Lipase in Liquid Emulsion Membrane for Downstream Processing
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Reverse Micelles-Mediated Transport of Lipase in Liquid Emulsion Membrane for Downstream Processing

机译：胶束逆向胶束介导的液体乳化膜在下游加工中的运输

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摘要

This work deals with the downstream processing of lipase (EC 3.1.1.3, from Aspergillus niger) using liquid emulsion membrane (LEM) containing reverse micelles for the first time. The membrane phase consisted of surfactants [cetyltrimethylammonium bromide (CTAB) and Span 80] and cosolvents (isooctane and paraffin light oil). The various process parameters for the extraction of lipase from aqueous feed were optimized to maximize activity recovery and purification fold. The mechanism of lipase transport through LEM consisted of three steps namely solubilization of lipase in reverse micelles, transportation of reverse micelles loaded with lipase through the liquid membrane, and release of the lipase into internal aqueous phase. The results showed that the optimum conditions for activity recovery (78.6%) and purification (3.14-fold) were feed phase ionic strength 0.10 M NaCl and pH 9.0, surfactants concentration (Span 80 0.18 M and CTAB 0.1 M), volume ratio of organic phase to internal aqueous phase 0.9, ratio of membrane emulsion to feed volume 1.0, internal aqueous phase concentration 1.0 M KCl and pH 7.0, stirring speed 450 rpm, and contact time 15 min. This work indicated the feasibility of LEM for the downstream processing of lipase.
机译：这项工作首次使用含有反胶束的液体乳状液膜（LEM）处理脂肪酶的下游加工（来自黑曲霉的EC 3.1.1.3）。膜相由表面活性剂[十六烷基三甲基溴化铵（CTAB）和Span 80]和助溶剂（异辛烷和石蜡轻油）组成。优化了从含水饲料中提取脂肪酶的各种工艺参数，以最大限度地提高活性回收率和纯化倍数。脂酶通过LEM转运的机理包括三个步骤，即将脂酶溶解在反胶束中，将装载有脂酶的反胶束转运穿过液膜，以及将脂酶释放到内部水相中。结果表明，最佳的活性回收率（78.6％）和纯化（3.14倍）的条件是进料相离子强度0.10 M NaCl和pH 9.0，表面活性剂浓度（Span 80 0.18 M和CTAB 0.1 M），有机物的体积比相相对于内部水相为0.9，膜乳液与进料体积之比为1.0，内部水相浓度为1.0 M KCl和pH 7.0，搅拌速度为450 rpm，接触时间为15分钟。这项工作表明LEM在脂肪酶下游加工中的可行性。

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