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首页> 外文期刊>Biotechnology Progress >Phosphatidylcholine Enrichment with Medium Chain Fatty Acids by Immobilized Phospholipase Ax-Catalyzed Acidolysis
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Phosphatidylcholine Enrichment with Medium Chain Fatty Acids by Immobilized Phospholipase Ax-Catalyzed Acidolysis

机译：固定化磷脂酶Ax催化酸解富集中链脂肪酸的磷脂酰胆碱

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摘要

Phospholipids are a biologically and industrially important class of compounds whose physical properties can be improved for diverse applications by substitution of medium-chain fatty acids for their native fatty acid chains. In this study, phosphatidylcholine (PC) was enriched with medium-chain fatty acids (MCFAs) by acidolysis with phospholipase A1 (PLA1) immobilized on Duolite A568. Response surface methodology was employed to evaluate the effects of the molar ratio of substrates (PC to free MCFAs), enzyme loading, and reaction temperature on the incorporation of free MCFAs into PC and on PC recovery. Enzyme loading and molar ratio of substrates contributed positively, but temperature negatively, to the incorporation of free MCFAs into PC. Increases in enzyme loading and the molar ratio of PC to free MCFAs led to increased incorporation of the latter into the former, but increased temperature had the opposite effect. By contrast, an increase in enzyme loading led to decreased PC recovery. Increased temperature had also a negative effect on PC recovery. Optimal conditions for maximum incorporation and PC recovery were molar ratio of PC to free MCFAs of 1:16, enzyme loading of 16%, and 50°C. Under these conditions, the incorporation of free MCFAs was 41% and the PC recovery was 53%.
机译：磷脂是一类在生物学和工业上很重要的化合物，通过用中链脂肪酸代替天然脂肪酸链可以改善其物理性能，从而可用于多种应用。在这项研究中，磷脂酰胆碱（PC）通过固定在Duolite A568上的磷脂酶A1（PLA1）酸解富集了中链脂肪酸（MCFA）。响应面方法用于评估底物的摩尔比（PC与游离MCFAs），酶负载和反应温度对将游离MCFAs掺入PC和PC回收率的影响。酶的负载量和底物的摩尔比对将游离MCFA掺入PC起到正向作用，而对温度则负向贡献。酶载量的增加和PC与游离MCFA的摩尔比导致后者与前者的结合增加，但温度升高则相反。相反，酶负载的增加导致PC回收率降低。温度升高也对PC回收率产生负面影响。最大掺入量和PC回收率的最佳条件是PC与游离MCFA的摩尔比为1:16，酶负载率为16％和50°C。在这些条件下，游离MCFA的掺入率为41％，PC回收率为53％。

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