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首页> 外文期刊>World Journal of Microbiology & Biotechnology >Transcriptional regulator GntR of Brucella abortus regulates cytotoxicity, induces the secretion of inflammatory cytokines and affects expression of the type IV secretion system and quorum sensing system in macrophages
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Transcriptional regulator GntR of Brucella abortus regulates cytotoxicity, induces the secretion of inflammatory cytokines and affects expression of the type IV secretion system and quorum sensing system in macrophages

机译：Brucella abortus的转录调节器GNTR调节细胞毒性，诱导炎症细胞因子的分泌，并影响巨噬细胞IV型分泌系统和法定传感系统的表达

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摘要

The pathogenic mechanisms of Brucella are still poorly understood. GntR is a transcriptional regulator and plays an important role in the intracellular survival of Brucella. To investigate whether GntR is involved in the cytotoxicity of Brucella abortus (B. abortus), we created a 2308 Delta gntR mutant of B. abortus 2308 (S2308). Lactate dehydrogenase (LDH) cytotoxicity assays using a murine macrophage cell line (RAW 264.7) show that high-dose infection with the parental strain produces a high level of cytotoxicity to macrophages, but the 2308 Delta gntR mutant exhibits a very low level of cytotoxicity, indicating that mutation of GntR impairs the cytotoxicity of B. abortus to macrophages. After the macrophages are infected with 2308 Delta gntR, the levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interleukin-8 (IL-8) increase and are slightly higher than that for the S2308 infected group, indicating that the 2308 Delta gntR mutant could induce the secretion of inflammatory cytokines. The virulence factor detection experiments indicate that genes involved in the type IV secretion system (T4SS) and quorum sensing system (QSS) are down-regulated in 2308 Delta gntR. The lower levels of survival of 2308 Delta gntR under various stress conditions and the increased sensitivity of 2308 Delta gntR to polymyxin B suggest that GntR is a virulence factor and that deletion of gntR reduces of B. abortus to stress conditions. Taken together, our results demonstrate that GntR is involved in the cytotoxicity, virulence and intracellular survival of B. abortus during its infection.
机译：布鲁氏菌的致病机制仍然明白。 GNTR是一种转录调节因子，在布鲁氏菌的细胞内存活中起重要作用。为了探讨GNTR是否参与Brucella Abortus（B.Abortus）的细胞毒性，我们创建了B. Abortus 2308的2308ΔGNTR突变体（S2308）。使用鼠巨噬细胞系（RAW 264.7）的乳酸脱氢酶（LDH）细胞毒性测定表明，具有父母菌株的高剂量感染产生高水平的细胞毒性对巨噬细胞，但2308 delta gntr突变体表现出非常低的细胞毒性水平，表明GNTR的突变损害B. Abortus的细胞毒性至巨噬细胞。巨噬细胞感染2308ΔGNTR后，肿瘤坏死因子-α（TNF-α），白细胞介素-6（IL-6）和白细胞介素-8（IL-8）的水平增加，略高于S2308感染组，表明2308ΔGNTR突变体可诱导炎性细胞因子的分泌。毒力因子检测实验表明，IV型分泌系统（T4S）和法定传感系统（QSS）中涉及的基因在2308ΔGNTR中下调。在各种应激条件下，2308ΔGntr的较低水平和2308 delta Gntr对多元辛B的增加的敏感性表明，GNTR是一种毒力因子，并且GNTR的缺失减少了B. abortus至胁迫条件。我们的结果一起表明，GNTR在感染期间涉及B. abortus的细胞毒性，毒力和细胞内存活。

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  • 作者

    Li Zhiqiang; Wang Shuli; Zhang Hui; Zhang Jinliang; Xi Li; Zhang Junbo; Chen Chuangfu;

  • 作者单位

    Shangqiu Normal Univ Sch Biotechnol &

    Food Shangqiu 476000 Henan Peoples R China;

    Shangqiu Normal Univ Sch Biotechnol &

    Food Shangqiu 476000 Henan Peoples R China;

    Shihezi Univ Coll Anim Sci &

    Technol Shihezi 832003 Xinjiang Peoples R China;

    Shangqiu Normal Univ Sch Biotechnol &

    Food Shangqiu 476000 Henan Peoples R China;

    Shangqiu Normal Univ Sch Biotechnol &

    Food Shangqiu 476000 Henan Peoples R China;

    Tongren Univ Coll Biol Agr &

    Forestry Tongren 554300 Guizhou Peoples R China;

    Shihezi Univ Coll Anim Sci &

    Technol Shihezi 832003 Xinjiang Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

    Brucella; 2308 Delta gntR; Cytotoxicity; Virulence;

    机译：布鲁氏菌;2308 delta gntr;细胞毒性;毒力;

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