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Biocompatible Nanoparticles Trigger Rapid Bacteria Clustering

机译：生物相容性纳米颗粒触发细菌快速聚集

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This study reveals an exciting phenomenon of stimulated bacteria clustering. Rapid aggregation and microbial arrest are shown to occur in Escherichia coli solutions of neutral pH when chitosan nanoparticles with positive zeta potential are added. Because chitosan nanoparticles can easily be dispersed in aqueous buffers,the rapid clustering phenomenon requires only minuscule nanoparticle concentrations and will be critical in developing new methods for extricating bacterial pathogens. This work establishes the dominant role of electrostatic attraction in bacteria-nanoparticle interactions by varying the nanoparticle zeta potential from highly positive to strongly negative values,and by exploring concentration effects. For strongly negative nanoparticles,no clusters form,while aggregates are small and loose at intermediate conditions. In addition,optical density measurements indicate that over 90% of the suspended bacteria flocculate within seconds of being mixed with chitosan nanoparticles of a highly positive surface charge. Finally,the nanoparticles are significantly more efficient as a clustering agent compared to an equal mass of molecular chitosan in solution,as the bacteria-nanoparticle clusters formed are substantially larger. The bacteria-nanoparticle aggregation effect demonstrated here promises a rapid separation method for aiding pathogen detection and for flocculation of bacteria in fermentation processes.
机译：这项研究揭示了刺激细菌聚集的令人兴奋的现象。当添加具有正ζ电位的壳聚糖纳米颗粒时，在中性pH的大肠杆菌溶液中会发生快速聚集和微生物停滞。由于壳聚糖纳米颗粒可以很容易地分散在水性缓冲液中，因此快速聚集现象只需要微小的纳米颗粒浓度，对于开发新的细菌病原体提取方法至关重要。这项工作通过将纳米粒子zeta电位从高正值改变为强负值，并探索浓度效应，确立了静电吸引在细菌-纳米粒子相互作用中的主导作用。对于强负性纳米粒子，没有簇形成，而聚集体在中间条件下较小且疏松。另外，光学密度测量表明，超过90％的悬浮细菌在与具有高度正电荷的壳聚糖纳米颗粒混合后的数秒内发生絮凝。最后，与溶液中等质量的分子壳聚糖相比，纳米颗粒作为聚集剂的效率更高，因为形成的细菌-纳米颗粒簇明显更大。本文展示的细菌-纳米颗粒聚集效应有望为细菌分离过程中的病原体检测和絮凝提供一种快速分离方法。

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