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首页> 外文期刊>Biotechnology Progress >Influence of Different Magnetites on Properties of Magnetic Pseudomonas aeruginosa Immobilizates Used for Biosurfactant Production
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Influence of Different Magnetites on Properties of Magnetic Pseudomonas aeruginosa Immobilizates Used for Biosurfactant Production

机译：不同磁铁矿对生产生物表面活性剂的铜绿假单胞菌磁性固定剂性能的影响

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During the last decades, whole-cell immobilization has been used successfully in many bioprocesses. In particular, it is aimed at implementing continuous production processes, reaching higher production rates, and reusing the biocatalyst. In some cases, effective retention of immobilizates in the bioprocess is not feasible by membranes or sieves due to pore plugging or undesired losses of immobilizates. In the present publication, it is reported about the investigation of magnetic immobilizates of Pseudomonas aeruginosa for application in continuous biosurfactant production of rhamnolipids by foam fractionation and retention of entrained immobilizates by high-gradient magnetic separation from foam. Different materials and methods were tested with respect to important parameters, such as stability, diffusion properties or magnetic separation. Good magnetic separation of immobilizates was achieved at 5% (w/w) magnetite loading. Best results in terms of homogeneous embedding, good diffusion properties, and stability enhancement vis-d-vis pure alginate beads was achieved with alginate beads with embedded Bayoxide~R magnetite or MagPrep~R silica particles. Although polyurethane immobilizates showed higher stabilities compared with alginate beads, rhamno-lipid diffusion in immobilizates was superior in magnetic alginate beads. Regarding bead production, smaller immobilizates were achieved with suspension polymerization compared to droplet extrusion by the JetCutting~R technology. In total, magnetic immobilizates are a promising tool for an easier handling of biocatalysts in a continuous biological production process, but they have to be adapted to the current production task.
机译：在过去的几十年中，全细胞固定已成功用于许多生物过程中。特别地，其目的在于实施连续生产过程，达到更高的生产率，并再利用生物催化剂。在某些情况下，由于孔堵塞或不希望的固定剂损失，膜或筛子无法有效地将固定剂保留在生物过程中。在本公开中，报道了关于铜绿假单胞菌的磁性固定剂的研究，该磁性固定剂用于通过泡沫分级分离和通过从泡沫中的高梯度磁分离保留夹带的固定剂而连续生产鼠李糖脂的生物表面活性剂。针对重要参数（例如稳定性，扩散特性或磁分离）测试了不同的材料和方法。在5％（w / w）磁铁矿负载下实现了固定剂的良好磁分离。相对于纯藻酸盐珠而言，使用嵌入了Bayoxide〜R磁铁矿或MagPrep〜R二氧化硅颗粒的藻酸盐珠，可以获得均匀的嵌入，良好的扩散性能和相对于纯藻酸盐珠的稳定性增强方面的最佳结果。尽管聚氨酯固定剂与藻酸盐珠相比显示出更高的稳定性，但鼠李糖脂在固定剂中的扩散在磁性藻酸盐珠中更为优异。关于珠的生产，与通过JetCutting〜R技术进行的液滴挤出相比，通过悬浮聚合可以实现较小的固定化。总体而言，磁性固定剂是一种在连续生物生产过程中更易于处理生物催化剂的有前途的工具，但必须使其适应当前的生产任务。

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