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首页> 外文期刊>Biotechnology Progress >A Cell Leakproof PLGA-Collagen Hybrid Scaffold for Cartilage Tissue Engineering
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A Cell Leakproof PLGA-Collagen Hybrid Scaffold for Cartilage Tissue Engineering

机译：用于软骨组织工程的细胞防泄漏PLGA-胶原混合支架

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摘要

A cell leakproof porous poly(DL-lactic-co-glycolic acid) (PLGA)-collagen hybrid scaffold was prepared by wrapping the surfaces of a collagen sponge except the top surface for cell seeding with a bi-layered PLGA mesh. The PLGA-collagen hybrid scaffold had a structure consisting of a central collagen sponge formed inside a bi-layered PLGA mesh cup. The hybrid scaffold showed high mechanical strength. The cell seeding efficiency was 90.0% when human mesenchymal stem cells (MSCs) were seeded in the hybrid scaffold. The central collagen sponge provided enough space for cell loading and supported cell adhesion, while the bi-layered PLGA mesh cup protected against cell leakage and provided high mechanical strength for the collagen sponge to maintain its shape during cell culture. The MSCs in the hybrid scaffolds showed round cell morphology after 4 weeks culture in chondrogenic induction medium. Immu-nostaining demonstrated that type II collagen and cartilaginous proteoglycan were detected in the extracellular matrices. Gene expression analyses by real-time PCR showed that the genes encoding type II collagen, aggrecan, and SOX9 were upregulated. These results indicated that the MSCs differentiated and formed cartilage-like tissue when being cultured in the cell leak-proof PLGA-collagen hybrid scaffold. The cell leakproof PLGA-collagen hybrid scaffolds should be useful for applications in cartilage tissue engineering.
机译：通过用双层PLGA网包裹用于细胞接种的顶表面以外的胶原海绵的表面，来制备细胞防漏多孔聚（DL-乳酸-乙醇酸）（PLGA）-胶原杂合支架。 PLGA-胶原混合支架具有由在双层PLGA网杯内部形成的中央胶原海绵组成的结构。杂种支架显示出高机械强度。当将人间充质干细胞（MSC）接种到杂种支架中时，细胞接种效率为90.0％。中央胶原海绵为细​​胞加载提供了足够的空间并支持细胞粘附，而双层PLGA网眼杯则可防止细胞渗漏，并为胶原海绵提供了很高的机械强度，可在细胞培养期间保持其形状。在软骨诱导培养基中培养4周后，杂种支架中的MSC显示出圆形细胞形态。免疫染色表明在细胞外基质中检测到II型胶原蛋白和软骨蛋白聚糖。通过实时PCR进行的基因表达分析表明，编码II型胶原蛋白，聚集蛋白聚糖和SOX9的基因被上调。这些结果表明，当在细胞防漏PLGA-胶原杂交支架中培养时，MSCs分化并形成了软骨样组织。防泄漏PLGA-胶原蛋白混合支架应可用于软骨组织工程。

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