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Experimental and Theoretical Assessment of Maximum Productivities for the Microalgae Chlamydomonas reinhardtii in Two Different Geometries of Photobioreactors

机译:在两种不同几何形状的光生物反应器中,微藻莱茵衣藻最大生产力的实验和理论评估

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摘要

The validity of a simple, reliable, and useful recently published formula enabling to calculate the maximum volumetric biomass productivities in photobioreactors (PBRs) was investigated through the cultivation of the microalga Chlamydomonas reinhardtii. Experimental maximum kinetic performances accurately obtained in two different, artificially lightened torus-plane and cylindrical reactors having the same specific illuminated area confirmed the availability, power, and robustness of such formula. The predictive kinetic parameters previously proposed and validated with cyanobacteria were then proved general and robust in case of eukaryotic microalgae, as postulated in the founding article. In this case, an additional criterion requiring rigorous control of the working illuminated fraction y = 1 ± (15%) inside the reactor is demonstrated. For this, the usefulness and reliability of a generalized two-flux model accurately describing the radiation field inside turbid culture media of C. reinhardtii were also established in this article. These important results contribute to identify the main engineering factors governing light-limited PBRs functioning and then to clarify some misinterpretations widely reported in the literature. Together with the referenced previous work, this article gives a framework toward optimal conception of PBRs on a strong physical basis.
机译:通过微藻莱茵衣藻的培养,研究了一种简单,可靠和有用的最近发布的公式的有效性,该公式能够计算光生物反应器(PBR)中的最大体积生物量生产率。在具有相同比照面积的两个不同的人工减轻的圆环平面和圆柱形反应堆中准确获得的实验最大动力学性能证实了该公式的可用性,功率和耐用性。如创始文章所述,在真核微藻的情况下,先前证明并经蓝细菌验证的预测动力学参数被证明是通用且可靠的。在这种情况下,说明了需要严格控制反应堆内部工作照明分数y = 1±(15%)的其他标准。为此,本文还建立了精确描述莱茵衣藻混浊培养基内辐射场的广义两通模型的实用性和可靠性。这些重要的结果有助于确定控制光限制PBR功能的主要工程因素,然后阐明文献中广泛报道的一些误解。结合之前的参考工作,本文提供了一个在坚实的物理基础上优化PBR概念的框架。

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