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首页> 外文期刊>Human Pathology >Global quality assessment of liver allograft C4d staining during acute antibody-mediated rejection in formalin-fixed, paraffin-embedded tissue
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Global quality assessment of liver allograft C4d staining during acute antibody-mediated rejection in formalin-fixed, paraffin-embedded tissue

机译：急性抗体介导的甲醛固定式，石蜡包埋组织中肝同种异体移植C4D染色的全球质量评估

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Discussion of liver antibody-mediated rejection during the 2011, 2013, and 2015 Banff liver sessions raised concerns over reliability of complement fragment 4d (C4d) staining, precipitating a global survey followed by a tissue microarray staining quality assessment study among centers on formalin-fixed, paraffin-embedded tissue. Tissue microarray sections containing tissue plugs of resected native and allograft (with acute antibody-mediated rejection) liver, heart, and kidney (n = 33 total cores) were sent to 31 centers for C4d staining using local method(s) and pathologist scoring. Digital whole-slide images (n = 40) were then semiquantitatively scored by 7 experts for background, distribution, and intensity of portal vein and capillary, hepatic artery, sinusoidal, and central vein endothelia and portal and central stromal staining. Results showed that strong and diffuse portal vein and capillary C4d staining, as determined by both local and central pathologists, clearly distinguished allografts showing acute antibody-mediated rejection from native livers and from those with evidence of weaker donor-specific antibody. Downstream vascular endothelial cell C4d staining and assessment were more variable and difficult to identify. C4d staining in the majority of laboratories reliably detects acute liver allograft antibody-mediated rejection in formalin-fixed, paraffin-embedded tissues. Assessment should focus on portal veins and capillaries, sinusoids, and central veins present in peripheral core needle biopsies. C4d staining in one organ does not always translate to staining in another.
机译：讨论2011年，2013年和2015年班夫肝脏会议期间肝脏抗体介导的拒绝提出了对补体片段4D（C4D）染色的可靠性的担忧，促进了全球调查，然后在福尔马林固定的中间中心进行组织微阵列染色质量评估研究，石蜡嵌入组织。含有切除的天然和同种异体移植物（具有急性抗体介导的排斥）肝脏，心脏和肾脏（n = 33总核）的组织微阵列的部分被送到31个C4D染色的C4D染色和病理学家评分。然后，通过7个专家进行数字全幻灯片图像（n = 40），由7个静脉和毛细管，肝动脉，正弦和中央静脉内皮和门静脉和中央基质染色和中央基体染色来分解7个专家。结果表明，由局部和中央病理学医生确定的强硬和弥漫性门静脉和毛细管C4D染色，清楚地区的同种异体移植物，显示来自天然肝脏的急性抗体介导的抑制剂以及具有较弱的供体特异性抗体的人。下游血管内皮细胞C4D染色和评估更可变，难以识别。大多数实验室中的C4D染色可靠地检测急性肝同种异体移植抗体介导的助剂介导的石蜡包埋组织。评估应专注于门静脉和毛细血管，正弦曲线和中央静脉，存在于外围核心针活检中。一个器官中的C4D染色并不总是转化为另一种染色。

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