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Influence of Pluronic F68 on Oxygen Mass Transfer

机译：Pluronic F68对氧气传质的影响

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Pluronic F68 is one of the most used shear protecting additives in cell culture cultivations. It is well known from literature that such surface-active surfactants lower the surface tension at the gas-liquid interface, which influences the mass transfer. In this study, the effect of Pluronic F68 on oxygen mass transfer in aqueous solutions was examined. Therefore, the gassing in/gassing out method and bubble size measurements were used. At low concentrations of 0.02 g/L, a 50% reduction on mass transfer was observed for all tested spargers and working conditions. An explanation of the observed effects by means of Hig-bie's penetration or Dankwerts surface renewal theory was applied. It could be demonstrated that the suppressed movement of the bubble surface layer is the main cause for the significant drop down of the k_L a-values. For Pluronic F68 concentrations above 0.1 g/L, it was observed that it comes to changes in bubble appearance and bubble size strongly dependent on the sparger type. By using the bubble size measurement data, it could be shown that only small changes in mass transfer coefficient (k_L) take place above the critical micelle concentration. Further changes on overall mass transfer at higher Pluronic F68 concentrations are mainly based on increasing of gas holdup and, more importantly, by increasing of the surface area available for mass transfer.
机译：Pluronic F68是细胞培养中最常用的抗剪切添加剂之一。从文献中众所周知，这种表面活性表面活性剂降低了气-液界面处的表面张力，这影响了传质。在这项研究中，考察了Pluronic F68对水溶液中氧气质量转移的影响。因此，使用了放气/放气方法和气泡尺寸测量。在0.02 g / L的低浓度下，对于所有测试的喷射器和工作条件，传质均降低了50％。通过Hig-bie的渗透或Dankwerts表面更新理论对观察到的效果进行了解释。可以证明，气泡表面层的运动受到抑制是k_L a值显着下降的主要原因。对于高于0.1 g / L的Pluronic F68浓度，观察到气泡外观和气泡尺寸的变化强烈取决于喷射器的类型。通过使用气泡尺寸测量数据，可以表明，在临界胶束浓度以上，传质系数（k_L）仅发生很小的变化。在更高的Pluronic F68浓度下，总体传质的进一步变化主要是基于气体滞留量的增加，更重要的是，通过增加可用于传质的表面积。

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