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首页> 外文期刊>Biotechnology Progress >Comparisons of Five Saccharomyces cerevisiae Strains for Ethanol Production from SPORL-Pretreated Lodgepole Pine
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Comparisons of Five Saccharomyces cerevisiae Strains for Ethanol Production from SPORL-Pretreated Lodgepole Pine

机译：5种酿酒酵母菌株在SPORL预处理的Lodgepole Pine乙醇生产乙醇中的比较

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The performances of five yeast strains under three levels of toxicity were evaluated using hydrolysates from lodgepole pine pretreated by Sulfite Pretreatment to Overcome the Recalcitrance of Lignocelluloses (SPORL). The highest level of toxicity was represented by the whole pretreated biomass slurry, while intermediate toxicity was represented by the hydroly-sate with partial loading of pretreatment spent liquor. The zero toxicity was represented using the enzymatic hydrolysate produced from thoroughly washed SPORL lodgepole pine solids. The results indicate that strains D5A and YRH400 can tolerate the whole pretreated biomass slurry to produce 90.1 and 73.5% theoretical ethanol yield. Strains Y1528, YRH403, and FPL450 did not grow in whole hydrolysate cultures and were observed to have lower ethanol productivities than D5A and YRH400 on the hydrolysate with intermediate toxicity. Both YRH400 and YRH403 were genetically engineered for xylose fermentation but were not able to consume xylose efficiently in hydrolysate.
机译：使用亚硫酸盐预处理来克服木素纤维素的难降解性的来自五倍子松的水解产物评估了五种酵母菌株在三种毒性水平下的性能。最高的毒性水平是由整个预处理过的生物质浆液代表的，而中等毒性是由水解产物和部分预处理废液代表的。使用彻底洗涤过的SPORL黑毛松固体产生的酶解产物代表了零毒性。结果表明，菌株D5A和YRH400可以耐受整个预处理的生物质浆料，以产生90.1％和73.5％的理论乙醇产率。菌株Y1528，YRH403和FPL450在整个水解产物培养物中均未生长，并且在中等毒性下，与D5A和YRH400相比，它们的乙醇生产率较低。 YRH400和YRH403都经过了基因工程改造，可用于木糖发酵，但不能有效地消耗水解产物中的木糖。

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