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首页> 外文期刊>Alcohol >Extracellular dopamine levels are lower in the medial prefrontal cortex of alcohol-preferring rats compared to Wistar rats.
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Extracellular dopamine levels are lower in the medial prefrontal cortex of alcohol-preferring rats compared to Wistar rats.

机译：与Wistar大鼠相比，嗜醇类大鼠的内侧前额叶皮层中的细胞外多巴胺水平较低。

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Previous studies have identified deficiencies in the mesocorticolimbic dopamine (DA) systems of alcohol-preferring (P) rats. This study uses quantitative microdialysis to compare the extracellular levels of DA in the medial prefrontal cortex (MPF) of P rats and outbred Wistar rats and also compares the effects of systemic ethanol administration on DA levels in the MPF using traditional microdialysis. In experiment 1, male Wistar and P rats were implanted with loop-style microdialysis probes and later perfused at 0.5 microl/min with artificial cerebrospinal fluid for 120 min prior to five baseline (20-min) sample collections. Three concentrations (5, 10, and 20 nM) of DA were then perfused in random order for 100 min each. Samples (20-min) were collected and stored at -70 degrees C until assayed using high performance liquid chromatography/electrochemical detection (HPLC/EC), and the data were analyzed using the quantitative no-net-flux (NNF) method. In experiment 2, male Wistar and P rats were implanted with dialysis probes aimed at the MPF. After collecting four baseline samples, all rats were injected (i.p.) with one dose of either 0.9% saline or 2.0 g/kg ethanol. Microdialysis samples were collected at 20-min intervals and stored at -70 degrees C until analyzed by HPLC/EC. NNF microdialysis yielded significantly (P<.05) lower extracellular DA concentrations in the MPF of P rats compared to Wistar rats (2.0+/-0.4 vs. 4.8+/-0.4 nM, respectively). The extraction fractions were not different between the P and Wistar groups (69+/-3 vs. 65+/-3%, respectively). No significant change in extracellular DA levels was observed in P rats or Wistar rats after either saline or 2g/kg ethanol. The lower extracellular concentrations of DA in the MPF of P rats compared to Wistar rats, without a difference in the extraction fraction, suggest that DA neurotransmission is lower in the MPF of the P rat. This lower DA neurotransmission could be a result of reduced activity of the DA neurons projecting to the MPF, reduced excitatory or increased inhibitory tone occurring locally within the MPF, and/or reduced DA innervation to the MPF. The lack of effect of systemic EtOH administration on extracellular DA levels in the MPF suggests that unlike the mesolimbic DA system, the mesocortical DA system is not responsive to acute EtOH administration.
机译：先前的研究已经确定了偏好酒精的（P）大鼠的中皮层皮质多巴胺（DA）系统的缺陷。这项研究使用定量微透析来比较P大鼠和远距离Wistar大鼠内侧前额叶皮层（MPF）中DA的细胞外水平，并使用传统的微透析方法比较全身性乙醇给药对MPF中DA水平的影响。在实验1中，雄性Wistar和P大鼠植入环式微透析探针，然后以0.5微升/分钟的速度用人工脑脊液灌注120分钟，然后收集五个基线（20分钟）。然后以随机顺序灌注三种浓度（5、10和20 nM）的DA，每次100分钟。收集样品（20分钟）并保存在-70摄氏度下，直到使用高效液相色谱/电化学检测（HPLC / EC）进行分析为止，然后使用定量无网通量（NNF）方法分析数据。在实验2中，雄性Wistar和P大鼠植入了针对MPF的透析探针。收集四个基线样品后，向所有大鼠注射（腹腔注射）一剂0.9％盐水或2.0 g / kg乙醇。以20分钟的间隔收集微透析样品，并保存在-70摄氏度下，直到通过HPLC / EC分析为止。与Wistar大鼠相比，NNF微透析可显着（P <.05）降低P大鼠MPF的细胞外DA浓度（分别为2.0 +/- 0.4与4.8 +/- 0.4 nM）。 P组和Wistar组之间的提取率没有差异（分别为69 +/- 3对65 +/- 3％）。用盐水或2g / kg乙醇处理后，在P大鼠或Wistar大鼠中未观察到细胞外DA水平的显着变化。与Wistar大鼠相比，P大鼠MPF中DA的细胞外浓度较低，而提取率无差异，这表明P大鼠MPF中DA神经传递较低。较低的DA神经传递可能是由于投射到MPF的DA神经元的活性降低，兴奋性降低或MPF局部发生的抑制音增加和/或DA对MPF的神经支配减少所致。缺乏全身性EtOH给药对MPF中细胞外DA水平的影响表明，与中脑边缘DA系统不同，中皮层DA系统对急性EtOH给药没有反应。

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