Development of a high-performance stationary phase is an essential demand for high-speed separation of protiens by liquid chromatography. Based on a novel porogenic mode, that is, using superfine granules of calcium carbonate as solid poroge and a mixture of cyclohexanol and dodecanol as liquid porogen, a rigid spherical biporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) matrix has been prepared by radical suspension-polymerization. The epoxide groups of the matrix were modfiied with diethylamine to afford the ionizable weak base 1-N,N-diethylamino-2-hydeoxypropy functionalities that are required for ion exchange chromatography. Results from scanning electron microscopy and mercury intrusion porosimetry measurements revealed that the matrix contained two families of pores, that is, micropores (10-90 nm) and macropores (180-4000 nm). Furthermore, the biporous medium possesses specific surface area as high as 91.3 m~2/g. Becasue of the presence of the macropores that provided convective flow channels for the mobile phase, the dynamic adsorption capacity was found to be as high as 5.46 mg/g wet bead at 300 cm/h, approximately 63.2% of its static capacity. In addition, the column efficiency and dynamic binding capacity decreased only slightly with mobile-phase flow rate in the range of 300-3000 cm/h. These properties made the packed bed with the bidisperse porous matrix suitable for high-speed protein chromatography.
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机译：高性能固定相的开发是通过液相色谱法快速分离蛋白质的基本要求。基于一种新颖的成孔方式，即以碳酸钙的超细颗粒为固体成孔剂，并以环己醇和十二烷醇的混合物作为液体成孔剂，通过自由基制备了刚性球形双孔聚甲基丙烯酸缩水甘油酯-二乙基丙烯酸乙烯酯共聚物。悬浮聚合。用二乙胺修饰基质的环氧基，以提供离子交换色谱所需的可电离的弱碱1-N，N-二乙氨基-2-乙氧基丙酸酯官能团。扫描电子显微镜和压汞法测量的结果表明，基质包含两个孔家族，即微孔（10-90 nm）和大孔（180-4000 nm）。此外，双孔介质具有高达91.3m 2 / g的比表面积。由于存在为流动相提供对流流动通道的大孔，因此动态吸附容量在300 cm / h时高达5.46 mg / g湿珠，约为其静态容量的63.2％。此外，当流动相流速在300-3000 cm / h范围内时，柱效和动态结合容量仅略有下降。这些特性使得具有双分散多孔基质的填充床适用于高速蛋白质色谱法。
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