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Study of the Attachment of Pseudomonas aeruginosa on Gold and Modified Gold Surfaces Using Surface Plasmon Resonance

机译：利用表面等离振子共振研究铜绿假单胞菌在金和修饰金表面上的附着

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摘要

This paper describes how the technique of surface plasmon resonance (SPR)can be utilized to follow (in real time)the attachment of Pseudomonas aeruginosa bacteria on bare gold and gold modified with a self-assembled monolayer (SAM)of mercap-tounadecanoic acid.We show that SPR is able to discriminate between the adsorption of live versus dead (thermally shocked)bacteria.Moreover,the SPR distinguishes between the adsorption of wild-type versus mutant bacteria (single gene knockouts),the concentration of the bacterial suspension,and between bacteria adsorbing on SAM-modified and bare gold.SPR is able to measure bacterial adsorption within seconds of the bacterial suspension being introduced.Finally,a qualitative correlation between results from SPR with a crystal violet staining assay for different mutant bacteria was observed.
机译：本文介绍了如何利用表面等离振子共振（SPR）技术实时跟踪铜绿假单胞菌细菌在裸金和巯基-十二碳烯酸自组装单层（SAM）修饰的金上的附着。我们证明SPR能够区分活细菌与死细菌（热冲击）之间的吸附。此外，SPR可以区分野生型与突变型细菌（单个基因敲除）的吸附，细菌悬浮液的浓度以及SPR能够在引入细菌悬浮液后的数秒内测量细菌的吸附。最后，观察到SPR结果与结晶紫染色法对不同突变细菌的定性相关性。

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